MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN GENES ISOLATED FROM DIFFERENT CLINICAL SOURCES USING MULTIPLEX PCR

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VALIZADEH E.; AMINI K.

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Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2016 | Volume:18 | Issue:12 Page(s): 26-32

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Title

MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN GENES ISOLATED FROM DIFFERENT CLINICAL SOURCES USING MULTIPLEX PCR

Author(s)

VALIZADEH E. | AMINI K. | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUREUSQ2

ENTEROTOXIN GENEQ1

MULTIPLEX POLYMERASE CHAIN REACTIONQ2

FOOD POISONINGQ2

Abstract

BACKGROUND AND OBJECTIVE: STAPHYLOCOCCUS AUREUS is one of the most common causes of FOOD POISONING in the world. Various studies indicated that 18-15 percent aureus strains isolated from different sources are be able to produce enterotoxin which known as the main factor of poisoning. The aim of this study was to identify ENTEROTOXIN GENEs, such as: (femA, see, sed, sec, seg, seb, sea) in S. aureus confirmed using multiple PCR method.METHODS: In this study, 60 samples of STAPHYLOCOCCUS AUREUS isolated from purulent infections, skin and have symptoms of poisoning: vomiting and diarrhea in humans, production of enterotoxin was examined. After DNA extraction of isolates, multiple PCR using specific primers for ENTEROTOXIN GENEs was performed.FINDINGS: Overall, 50% of STAPHYLOCOCCUS AUREUS isolates contain one or more ENTEROTOXIN GENE. The most abundant gene was sea (30%) and sed (10%), see (3.8%), sec (6.1%) were also identified.CONCLUSION: It was found that other genes such as TSST-1 are involved STAPHYLOCOCCUS AUREUS enterotoxin production in the creation of visual acuity in addition to above genes. In general, the presence of STAPHYLOCOCCUS AUREUS in human clinical specimens, especially enterotoxigenic strains, can be considered a potential risk for health

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APA: Copy

VALIZADEH, E., & AMINI, K.. (2016). MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN GENES ISOLATED FROM DIFFERENT CLINICAL SOURCES USING MULTIPLEX PCR. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 18(12), 26-32. SID. https://sid.ir/paper/73622/en

Vancouver: Copy

VALIZADEH E., AMINI K.. MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN GENES ISOLATED FROM DIFFERENT CLINICAL SOURCES USING MULTIPLEX PCR. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2016;18(12):26-32. Available from: https://sid.ir/paper/73622/en

IEEE: Copy

E. VALIZADEH, and K. AMINI, “MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN GENES ISOLATED FROM DIFFERENT CLINICAL SOURCES USING MULTIPLEX PCR,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 18, no. 12, pp. 26–32, 2016, [Online]. Available: https://sid.ir/paper/73622/en

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