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Information Journal Paper

Title

STUDY ON EFFECTIVE COMPONENTS ON THE QUANTITY AND QUALITY OF GENOMIC DNA EXTRACTED FROM IRAN CYCLAMEN SPECIES

Pages

  11-16

Abstract

 GENOMIC DNA EXTRACTION with a high quantity and quality is a basic requerment in molecular biology. DNA extraction from plant tissue because of presence of polysaccharids, tanens, phenolic compounds and proteins faced with some problems that negatively affect the DNA quality. DNA extraction methods should be identified to reduce these compounds. In order to select the best GENOMIC DNA EXTRACTION method from CYCLAMEN tissues, four DNA extraction methodsluded (1) Murry and Thampson (1980), (2) Doyle and Doyle (1990), (3) Dellaporta et al (1983) and (4) Lodhi et al (1994) were compared. Young and well expanded mature leaves were used for DNA extraction. Quantity and quality of extracted genomic DNA were compared by applying the spectophotometric, agarose gel electrophoresis, treatment with restriction endonuclease (EcoRI) and polymerase chain rection (PCR). Ultimately, considering the quantity and quality of DNA and the results of PCR, the method of Murry and Thampson (1980) was recomended for high pure DNA extraction in this ornamental plant. Highest amount of DNA 395 ng/µl (79 µg/g) was obtained from 1.0 g young tissue with good quality.

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    APA: Copy

    ALAEI, M., NADERI, R., KHALIGHI, A., & SALAMI, S.A.R.. (2007). STUDY ON EFFECTIVE COMPONENTS ON THE QUANTITY AND QUALITY OF GENOMIC DNA EXTRACTED FROM IRAN CYCLAMEN SPECIES. PAJOUHESH-VA-SAZANDEGI, 19(4 (73 IN AGRONOMY AND HORTICULTURE)), 11-16. SID. https://sid.ir/paper/19969/en

    Vancouver: Copy

    ALAEI M., NADERI R., KHALIGHI A., SALAMI S.A.R.. STUDY ON EFFECTIVE COMPONENTS ON THE QUANTITY AND QUALITY OF GENOMIC DNA EXTRACTED FROM IRAN CYCLAMEN SPECIES. PAJOUHESH-VA-SAZANDEGI[Internet]. 2007;19(4 (73 IN AGRONOMY AND HORTICULTURE)):11-16. Available from: https://sid.ir/paper/19969/en

    IEEE: Copy

    M. ALAEI, R. NADERI, A. KHALIGHI, and S.A.R. SALAMI, “STUDY ON EFFECTIVE COMPONENTS ON THE QUANTITY AND QUALITY OF GENOMIC DNA EXTRACTED FROM IRAN CYCLAMEN SPECIES,” PAJOUHESH-VA-SAZANDEGI, vol. 19, no. 4 (73 IN AGRONOMY AND HORTICULTURE), pp. 11–16, 2007, [Online]. Available: https://sid.ir/paper/19969/en

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