EVALUATION OF THREE DNA EXTRACTION METHODS FOR DETECTION OF BRUCELLA DNA IN HUMAN SERUM SAMPLES

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PEERIDOGAHEH H.; VALINEJAD Z.; POURFARZI F.

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Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2012 | Volume:14 | Issue:6 (SUPPL 3) Page(s): 40-48

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Title

EVALUATION OF THREE DNA EXTRACTION METHODS FOR DETECTION OF BRUCELLA DNA IN HUMAN SERUM SAMPLES

Author(s)

PEERIDOGAHEH H. | VALINEJAD Z. | POURFARZI F. | Issue Writer Certificate

Keywords

BRUCELLOSISQ2

DNA EXTRACTIONQ2

PCRQ2

Abstract

Background: Human BRUCELLOSIS is a significant public health concern in many countries, including Iran. Therefore, the development of new diagnostic techniques, with high sensitivity and minimum risk of laboratory infection are of great importance. PCR is one of the procedures which has these advantages. However, PCR efficiency is largely dependent on DNA EXTRACTION methods. In this study, we studied the efficiency of three different extraction methods of brucella DNA in serum samples.Materials and Methods: In this experimental study, microbial suspensions were initially prepared in saline that its turbidity was equivalent to 0.5 McFarland. Human serum samples were spiked with certain concentrations of Brucella melitensis in vitro. DNA was extracted by three methods and tested by a genus-specific PCR method.Results: Our results showed that the cinneagen kit protocol detected brucella DNA in lower serum concentrations compared with the other protocols. Cinnagen kit could detect brucella DNA in ten-fold dilution in comparison with the other two methods.Conclusion: According to the findings of this study, cinnagen kit was the preferred assay method that yields a better sensitivity for isolation of brucella DNA in serum samples.

Cites

OPTIMIZATION OF A MULTIPLEX PCR FOR SIMULTANEOUS DETECTION OF FOODBORNE PATHOGENS SALMONELLA SPP. AND ESCHERICHIA COLI O157:H7 AND CONTAMINATION PREVALENCE ASSAY IN MEAT PRODUCTS

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APA: Copy

PEERIDOGAHEH, H., VALINEJAD, Z., & POURFARZI, F.. (2012). EVALUATION OF THREE DNA EXTRACTION METHODS FOR DETECTION OF BRUCELLA DNA IN HUMAN SERUM SAMPLES. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 14(6 (SUPPL 3)), 40-48. SID. https://sid.ir/paper/68968/en

Vancouver: Copy

PEERIDOGAHEH H., VALINEJAD Z., POURFARZI F.. EVALUATION OF THREE DNA EXTRACTION METHODS FOR DETECTION OF BRUCELLA DNA IN HUMAN SERUM SAMPLES. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2012;14(6 (SUPPL 3)):40-48. Available from: https://sid.ir/paper/68968/en

IEEE: Copy

H. PEERIDOGAHEH, Z. VALINEJAD, and F. POURFARZI, “EVALUATION OF THREE DNA EXTRACTION METHODS FOR DETECTION OF BRUCELLA DNA IN HUMAN SERUM SAMPLES,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 14, no. 6 (SUPPL 3), pp. 40–48, 2012, [Online]. Available: https://sid.ir/paper/68968/en

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