MOLECULAR IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY RD-TYPING AND PCR-RFLP METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TOURABI N.; MOSAVARI N.; NAZARI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Veterinary Research & Biological Products Year:2017 | Volume:30 | Issue:3 (116) Page(s): 16-25

Download Full-Text

Persian Verion

View:

927

Download:

Cites:

Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY RD-TYPING AND PCR-RFLP METHOD

Author(s)

TOURABI N. | MOSAVARI N. | NAZARI R. | Issue Writer Certificate

Keywords

MYCOBACTERIUM TUBERCULOSIS COMPLEXQ1

RD-TYPINGQ1

PCR 16S RRNAQ1

IS6110Q1

Abstract

Tuberculosis is still considered as one of the most important zoonotic diseases and the cause of infectious death and human casualties. The main purpose of this study was to set up and diagnose MYCOBACTERIUM TUBERCULOSIS COMPLEX isolates in patients of FARS, Shiraz using the Regions of Difference (RD) typing and RFLP techniques. The genomes of 50 isolates were extracted by boiling method. To determine the genus of the isolates, PCR technique based on 16 s rRNA was used. IS6110 PCR and RD typing were used for the detection of MYCOBACTERIUM TUBERCULOSIS COMPLEX. Finally, the PCR-RFLP method was used as a comparing method for comparative evaluation of RD typing to identify mycobacteria. Amplification of 543 bp bands in 16s rRNA PCR showed that all studied isolates belonged to the genus Mycobacterium. In IS6110 gene PCR, it was shown that all isolates were MYCOBACTERIUM TUBERCULOSIS COMPLEX due to the amplification of 245 bp bands. In RD typing the identification was based on RD1, RD4, RD9 and RD12. The comparative method of PCR-RFLP on pseudo gene oxyR showed that all isolates were Mycobacterium tuberculosis. Using RD-TYPING, it was found that all isolates achieved from Shiraz were Mycobacterium tuberculosis. The absence of Mycobacterium bovisisolates represents good hygiene and consumption of pasteurized milk in this city. The results of this study also reflect the ability of RD typing technique in differentiation of mycobacteria.

Cites

No record.

References

No record.

Cite

APA: Copy

TOURABI, N., MOSAVARI, N., & NAZARI, R.. (2017). MOLECULAR IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY RD-TYPING AND PCR-RFLP METHOD. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 30(3 (116)), 16-25. SID. https://sid.ir/paper/200880/en

Vancouver: Copy

TOURABI N., MOSAVARI N., NAZARI R.. MOLECULAR IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY RD-TYPING AND PCR-RFLP METHOD. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2017;30(3 (116)):16-25. Available from: https://sid.ir/paper/200880/en

IEEE: Copy

N. TOURABI, N. MOSAVARI, and R. NAZARI, “MOLECULAR IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX BY RD-TYPING AND PCR-RFLP METHOD,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 30, no. 3 (116), pp. 16–25, 2017, [Online]. Available: https://sid.ir/paper/200880/en

Related Journal Papers