MOLECULAR DIFFERENTIATION BETWEEN BOVINE MYCOBACTERIUM BOVIS ISOLATES AND HUMAN MYCOBACTERIUM TUBERCULOSIS ISOLATES IN IRAN THROUGH PCR-RFLP METHOD AND COMPARISON WITH 5 STANDARD STRAINS

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MOSAVARI N.; SALEHI MITRA; TADAYON K.; MOHAMMAD TAHERI MOHAMMAD; SOLEYMANI KIOUMARS; AREF PAZHOUHI R.; SEPEHRI SERESHT S.; ALAVI M.

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2008 | Volume:2 | Issue:1 Page(s): 1-7

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Title

MOLECULAR DIFFERENTIATION BETWEEN BOVINE MYCOBACTERIUM BOVIS ISOLATES AND HUMAN MYCOBACTERIUM TUBERCULOSIS ISOLATES IN IRAN THROUGH PCR-RFLP METHOD AND COMPARISON WITH 5 STANDARD STRAINS

Author(s)

Keywords

MYCOBACTERIUM BOVISQ4

MYCOBACTERIUM TUBERCULOSIS COMPLEXQ4

OXYR PSEUDOGENEQ4

PCR-RFLPQ2

ALUIQ4

Abstract

Background and Objectives: MYCOBACTERIUM TUBERCULOSIS COMPLEX is consisted of homogenous organisms. They are slowly growing mycobacteria and their isolation and identification are difficult and time consuming. Differentiation of MYCOBACTERIUM BOVIS, causative mammalian tuberculosis, from other members of MYCOBACTERIUM TUBERCULOSIS COMPLEX is very important in epidemiology and control of disease in humans and animals. The aim of this study was to evaluate a molecular method to differentiate Mycobacteriom bovis from Mycobacterium tuberculosis.Material and Methods: DNA human isolates of Mycobacterium tuberculosis (n=6) and MYCOBACTERIUM BOVIS isolates (50) were extracted and used as template in PCR. A 548bp fragment of OXYR PSEUDOGENE was amplified and digested with ALUI endo nuclease. The nucleotide 285 could be adenine (M. bovis) or guanine (M. tuberculosis). Such variation produces different restriction site for ALUI.Results: There were three incisive fragments in all MYCOBACTERIUM BOVIS and MYCOBACTERIUM BOVIS BCG strains and one incisive fragment in other members of MYCOBACTERIUM TUBERCULOSIS COMPLEX.Conclusion: PCR-RFLP method on 548bp fragment of oxyR gene is a rapid and accurate method to differentiate MYCOBACTERIUM BOVIS and MYCOBACTERIUM BOVIS BCG from other members of MYCOBACTERIUM TUBERCULOSIS COMPLEX.

Cites

DNA FINGERPRINTING OF MYCOBACTERIUM BOVIS AN5 STRAIN BY RFLP TECNIQUE

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APA: Copy

MOSAVARI, N., SALEHI, MITRA, TADAYON, K., MOHAMMAD TAHERI, MOHAMMAD, SOLEYMANI, KIOUMARS, AREF PAZHOUHI, R., SEPEHRI SERESHT, S., & ALAVI, M.. (2008). MOLECULAR DIFFERENTIATION BETWEEN BOVINE MYCOBACTERIUM BOVIS ISOLATES AND HUMAN MYCOBACTERIUM TUBERCULOSIS ISOLATES IN IRAN THROUGH PCR-RFLP METHOD AND COMPARISON WITH 5 STANDARD STRAINS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2(1), 1-7. SID. https://sid.ir/paper/132660/en

Vancouver: Copy

MOSAVARI N., SALEHI MITRA, TADAYON K., MOHAMMAD TAHERI MOHAMMAD, SOLEYMANI KIOUMARS, AREF PAZHOUHI R., SEPEHRI SERESHT S., ALAVI M.. MOLECULAR DIFFERENTIATION BETWEEN BOVINE MYCOBACTERIUM BOVIS ISOLATES AND HUMAN MYCOBACTERIUM TUBERCULOSIS ISOLATES IN IRAN THROUGH PCR-RFLP METHOD AND COMPARISON WITH 5 STANDARD STRAINS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2008;2(1):1-7. Available from: https://sid.ir/paper/132660/en

IEEE: Copy

N. MOSAVARI, MITRA SALEHI, K. TADAYON, MOHAMMAD MOHAMMAD TAHERI, KIOUMARS SOLEYMANI, R. AREF PAZHOUHI, S. SEPEHRI SERESHT, and M. ALAVI, “MOLECULAR DIFFERENTIATION BETWEEN BOVINE MYCOBACTERIUM BOVIS ISOLATES AND HUMAN MYCOBACTERIUM TUBERCULOSIS ISOLATES IN IRAN THROUGH PCR-RFLP METHOD AND COMPARISON WITH 5 STANDARD STRAINS,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 2, no. 1, pp. 1–7, 2008, [Online]. Available: https://sid.ir/paper/132660/en

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