COMPARATIVE EVALUATION OF DIFCO BRUCELLA AGAR (BD) AND GLYCEROL DEXTROSE AGAR CULTURE MEDIA FOR PRODUCTION OF BRUCELLOSIS REV 1 VACCINE

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ALAMIAN S.

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Journal Paper

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Journal: Veterinary Research & Biological Products Year:2015 | Volume:28 | Issue:1(106) Page(s): 18-23

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Title

COMPARATIVE EVALUATION OF DIFCO BRUCELLA AGAR (BD) AND GLYCEROL DEXTROSE AGAR CULTURE MEDIA FOR PRODUCTION OF BRUCELLOSIS REV 1 VACCINE

Author(s)

ALAMIAN S. | Issue Writer Certificate

Keywords

BRUCELLA AGARQ2

GLYCEROL DEXTROSE AGARQ2

VACCINEQ2

REV 1Q2

BRUCELLOSISQ2

Abstract

Control and eradication of the BRUCELLOSIS is loosed on vaccination of susceptible animals by REV 1 VACCINE. Production of this VACCINE is done by BRUCELLA AGAR culture media that is imported. Recently, the price of the medium has increased because of economic sanctions. Therefore, regarding the production of GLYCEROL DEXTROSE AGAR in Iran and its record in international sources, this study was done for possible replacement with this medium. So, 10 batches of VACCINE were produced using this medium and they were compared with the VACCINEs produced by BRUCELLA AGAR with the same master seed. The number of live bacteria in 1 ml of bulk product was counted by counting the number of colony forming unit (CFU). In addition, other quality tests like morphology of colony or determination of the percentage of rough organisms, safety and efficacy test were done on each batch. The results indicated the mean numbers of live bacteria in 1 ml of bulk products was134.37×109 and 118.55×109 (CFU) and the percentage of rough colonies was %0.75 and %1.281 for VACCINEs produced by BRUCELLA AGAR and GLYCEROL DEXTROSE AGAR respectively. There was not any difference in other quality tests includes safety and efficacy and all quality parameters remained within the standard ranges. Thus regarding the suitable results, this new culture medium can be used for Rev1 VACCINE production.

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APA: Copy

ALAMIAN, S.. (2015). COMPARATIVE EVALUATION OF DIFCO BRUCELLA AGAR (BD) AND GLYCEROL DEXTROSE AGAR CULTURE MEDIA FOR PRODUCTION OF BRUCELLOSIS REV 1 VACCINE. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 28(1(106)), 18-23. SID. https://sid.ir/paper/200888/en

Vancouver: Copy

ALAMIAN S.. COMPARATIVE EVALUATION OF DIFCO BRUCELLA AGAR (BD) AND GLYCEROL DEXTROSE AGAR CULTURE MEDIA FOR PRODUCTION OF BRUCELLOSIS REV 1 VACCINE. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2015;28(1(106)):18-23. Available from: https://sid.ir/paper/200888/en

IEEE: Copy

S. ALAMIAN, “COMPARATIVE EVALUATION OF DIFCO BRUCELLA AGAR (BD) AND GLYCEROL DEXTROSE AGAR CULTURE MEDIA FOR PRODUCTION OF BRUCELLOSIS REV 1 VACCINE,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 28, no. 1(106), pp. 18–23, 2015, [Online]. Available: https://sid.ir/paper/200888/en

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