COMPARISON OF PARAFFIN BAIT, HUMIC ACID VITAMIN B AGAR AND PARAFFIN AGAR METHODS TO ISOLATE NOCARDIA FROM SOIL

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RASOULINASAB M.; HABIBNIA SH.; HEIDARIEH P.; POURMAND M.R.; FATAHI M.; ESHRAGHI S.

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Journal: MEDICAL LABORATORY JOURNAL Year:2013 | Volume:7 | Issue:SUPPL. (5) Page(s): 29-36

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Title

COMPARISON OF PARAFFIN BAIT, HUMIC ACID VITAMIN B AGAR AND PARAFFIN AGAR METHODS TO ISOLATE NOCARDIA FROM SOIL

Author(s)

Keywords

NOCARDIAQ2

SOILQ3

PARAFFIN BAITINGQ2

HUMIC ACID VITAMIN BQ2

Abstract

Background and Objective: The Isolation of NOCARDIA species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for NOCARDIA species.Material and Methods: The SOIL samples were collected from different regions of Tehran province, Iran, and carefully transferred to the laboratory. The samples were cultured in three different media including PARAFFIN BAITING, HUMIC ACID VITAMIN B agar and Paraffin agar, and incubated for 3-4 weeks at 35oC.Results: Of 110 SOIL samples, 31 NOCARDIA isolates (28.18%) were obtained from the media including PARAFFIN BAITING, (19; 17.27%), Humic acid and vitamin B agar (4; 3.63%), and Paraffin agar, (8; 7.27%).Conclusion: because of high rate of isolation, low cost and the clearance of colonies suspected NOCARDIA, Paraffin Bait technique is more reliable and efficient compared to the other methods.

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APA: Copy

RASOULINASAB, M., HABIBNIA, SH., HEIDARIEH, P., POURMAND, M.R., FATAHI, M., & ESHRAGHI, S.. (2013). COMPARISON OF PARAFFIN BAIT, HUMIC ACID VITAMIN B AGAR AND PARAFFIN AGAR METHODS TO ISOLATE NOCARDIA FROM SOIL. MEDICAL LABORATORY JOURNAL, 7(SUPPL. (5)), 29-36. SID. https://sid.ir/paper/409623/en

Vancouver: Copy

RASOULINASAB M., HABIBNIA SH., HEIDARIEH P., POURMAND M.R., FATAHI M., ESHRAGHI S.. COMPARISON OF PARAFFIN BAIT, HUMIC ACID VITAMIN B AGAR AND PARAFFIN AGAR METHODS TO ISOLATE NOCARDIA FROM SOIL. MEDICAL LABORATORY JOURNAL[Internet]. 2013;7(SUPPL. (5)):29-36. Available from: https://sid.ir/paper/409623/en

IEEE: Copy

M. RASOULINASAB, SH. HABIBNIA, P. HEIDARIEH, M.R. POURMAND, M. FATAHI, and S. ESHRAGHI, “COMPARISON OF PARAFFIN BAIT, HUMIC ACID VITAMIN B AGAR AND PARAFFIN AGAR METHODS TO ISOLATE NOCARDIA FROM SOIL,” MEDICAL LABORATORY JOURNAL, vol. 7, no. SUPPL. (5), pp. 29–36, 2013, [Online]. Available: https://sid.ir/paper/409623/en

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