PSY IS A RATE-LIMITING ENZYME IN CAROTENOID BIOSYNTHESIS PATHWAY IN MAIZE (ZEA MAYS) LEAVES

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AHMADABADI MOHAMMAD

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Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2014 | Volume:4 | Issue:13 Page(s): 71-75

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Title

PSY IS A RATE-LIMITING ENZYME IN CAROTENOID BIOSYNTHESIS PATHWAY IN MAIZE (ZEA MAYS) LEAVES

Author(s)

AHMADABADI MOHAMMAD | Issue Writer Certificate

Keywords

MAIZEQ2

CAROTENOIDSQ2

GENETIC ENGINEERINGQ2

PHYTOENE SYNTHASEQ1

Abstract

Aim and Background: CAROTENOIDS are one of the most abundant pigments in nature and an essential compound of photosynthesis apparatus. In addition, CAROTENOIDS play a significant role in human and animal diet, because they are precursors for vitamin A and act as antioxidants. Thus, increasing carotenoid levels in plants, especially cereals as the main food for human and animals, could have a dramatic impact on improving plant yield and nutritional value. Among several enzymes involved in carotenoid biosynthesis pathway, PSY has been known as a key enzyme. So far, three PSY genes have been identified in MAIZE; however, all related enzymes do not show similar activity in all tissues. Considering existing of three PSY genes, whether over-expression of this enzyme can still increase carotenoid biosynthesis levels in MAIZE, is largely unknown.Materials and methods: Tissue culture and transformation was carried out using the method developed by Ahmadabadi et al. (1). Total DNA was extracted from MAIZE leaves according to Doyle & Doyle method (5). To confirm transgene presence in regenerated transgenic plants, PCR reaction was performed using specific primers. Pigment content of transgenic plants was analyzed by HPLC method.Results: In this study, through expression of a foreign PSY gene in MAIZE, we showed that MAIZE include a high potential to increase carotenoid levels via over-expression of PSY, and thus, the PSY gene is a rate-limiting enzyme in carotenoid biosynthesis pathway in MAIZE. It should be mentioned that transgenic plants were similar to wild-type plants in terms of growth, development and yield, while the general carotenoid levels was significantly increased. In particle, the level of beta-carotene as a precursor of vitamin A was improved considerably.Conclusion: Our results show that MAIZE has a high potential for increasing carotenoid levels, especially beta-carotene, that can have a significant role in MAIZE breeding programs in order to improve its nutritional value without any negative effect on yield quantity.

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APA: Copy

AHMADABADI, MOHAMMAD. (2014). PSY IS A RATE-LIMITING ENZYME IN CAROTENOID BIOSYNTHESIS PATHWAY IN MAIZE (ZEA MAYS) LEAVES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 4(13), 71-75. SID. https://sid.ir/paper/204372/en

Vancouver: Copy

AHMADABADI MOHAMMAD. PSY IS A RATE-LIMITING ENZYME IN CAROTENOID BIOSYNTHESIS PATHWAY IN MAIZE (ZEA MAYS) LEAVES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2014;4(13):71-75. Available from: https://sid.ir/paper/204372/en

IEEE: Copy

MOHAMMAD AHMADABADI, “PSY IS A RATE-LIMITING ENZYME IN CAROTENOID BIOSYNTHESIS PATHWAY IN MAIZE (ZEA MAYS) LEAVES,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 4, no. 13, pp. 71–75, 2014, [Online]. Available: https://sid.ir/paper/204372/en

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