PREPARATION OF ARCHAEOSOME ADJUVANT FROM MEMBRANE POLAR LIPIDS OF LARGE SCALE CULTURED METHANOBREVEIBACTER SMITHII

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SHARIFAT SALMANI ALI; AGHASADEGHI MOHAMMAD REZA; NATEGH RAKHSHANDEH; MOKHTARI AZAD TALAT; SIADAT SEYED DAVAR

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2014 | Volume:5 | Issue:15 Page(s): 51-59

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Title

PREPARATION OF ARCHAEOSOME ADJUVANT FROM MEMBRANE POLAR LIPIDS OF LARGE SCALE CULTURED METHANOBREVEIBACTER SMITHII

Author(s)

Keywords

ADJUVANTQ2

ARCHAEOSOMEQ2

POLAR LIPIDSQ2

METHANOBREVIBACTER SMITHIIQ2

Abstract

Aim and Background: Archaeal lipid derived liposomes referred to as ARCHAEOSOMEs are among newly studied ADJUVANTs with some specific immunopotent properties. In the present study large scale cultivation of METHANOBREVIBACTER SMITHII, lipid extraction of obtained biomass and ARCHAEOSOME production was assessed and evaluated.Materials and Methods: archaea METHANOBREVIBACTER SMITHII, DSMZ 2375 was grown in the fermenter under controlled pH, temperature and anaerobic atmosphere condition to achieve archaeal cell mass. Total POLAR LIPIDS were extracted via methanol/chloroform/water (2:1:0.8 v/v) solution from the frozen and thawed archaeal cells obtained from large scale cultivation. Archaeal liposomes prepared through hydration of extracted POLAR LIPIDS by PBS buffer containing bovine serum albumin as the tentative antigen. Hydration was also carried out by BSA free PBS buffer to prepare antigen free ARCHAEOSOMEs to be compared with antigen containing type. Purity of extracted lipids evaluated via agarose gel electrophoresis and SDS-PAGE. Antigen concentration and entrapment analyzed by Lowry method and SDS-PAGE, respectively.Results: Analysis of extracted lipids and ARCHAEOSOMEs revealed the high concentration and purity of obtained total POLAR LIPIDS and successful entrapment of antigen in the ARCHAEOSOMEs.Conclusion: ADJUVANT application of ARCHAEOSOMEs obtained from total POLAR LIPIDS of METHANOBREVIBACTER SMITHII could be a promising achievement in a variety of vaccine studies.

Cites

Preparing Nanoarchaeosome Containing Triptorelin Acetate and Evaluation of Its Cellular Toxic Effect on PC3 Prostate Cancer Cell Line

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APA: Copy

SHARIFAT SALMANI, ALI, AGHASADEGHI, MOHAMMAD REZA, NATEGH, RAKHSHANDEH, MOKHTARI AZAD, TALAT, & SIADAT, SEYED DAVAR. (2014). PREPARATION OF ARCHAEOSOME ADJUVANT FROM MEMBRANE POLAR LIPIDS OF LARGE SCALE CULTURED METHANOBREVEIBACTER SMITHII. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 5(15), 51-59. SID. https://sid.ir/paper/204386/en

Vancouver: Copy

SHARIFAT SALMANI ALI, AGHASADEGHI MOHAMMAD REZA, NATEGH RAKHSHANDEH, MOKHTARI AZAD TALAT, SIADAT SEYED DAVAR. PREPARATION OF ARCHAEOSOME ADJUVANT FROM MEMBRANE POLAR LIPIDS OF LARGE SCALE CULTURED METHANOBREVEIBACTER SMITHII. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2014;5(15):51-59. Available from: https://sid.ir/paper/204386/en

IEEE: Copy

ALI SHARIFAT SALMANI, MOHAMMAD REZA AGHASADEGHI, RAKHSHANDEH NATEGH, TALAT MOKHTARI AZAD, and SEYED DAVAR SIADAT, “PREPARATION OF ARCHAEOSOME ADJUVANT FROM MEMBRANE POLAR LIPIDS OF LARGE SCALE CULTURED METHANOBREVEIBACTER SMITHII,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 5, no. 15, pp. 51–59, 2014, [Online]. Available: https://sid.ir/paper/204386/en

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