STABILITY AND ACTIVITY OF ALCOHOL DEHYDROGENASE IN THE PRESENCE OF KOSMOTROPE ANDCHAOTROPE SALT SERIES

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SHARIFI RASOUL; SOLEIMANI SALEH; AMINI NAZILA; SAMIRA SHARIFI; ATYABI SEYED MOHAMMAD; MIROLIAEI MEHRAN

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2014 | Volume:4 | Issue:14 Page(s): 73-78

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Title

STABILITY AND ACTIVITY OF ALCOHOL DEHYDROGENASE IN THE PRESENCE OF KOSMOTROPE ANDCHAOTROPE SALT SERIES

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Keywords

HORSELIVERALCOHOLDEHYDROGENASEQ2

ACTIVITY AND STABILITYQ2

HOFMEISTERSERIESQ2

KOSMOTROPEANDCHAOTROPEQ2

Abstract

Aim and Background: Enzyme action at high salt concentrations isofpractical relevance in a number of application areas.Both ACTIVITY AND STABILITY of the horse liveralcohol dehydrogenase correlatewell withtheHofmeister series in terms ofthe salt's kosmotropic/chaotropic properties, which areassessedby the Jones–Dole viscosity Bcoefficients (B+ for cationsandB− for anions).In this study thermal stability and activityofthe enzyme were investigated.Materials and Methods: Inthisstudy, the effects ofhigh concentrations (1- 4 M) ofNaCl, NaNO3, Na2SO4 NaClO4, NaAc and concentrations (1-3 M) ofKCl, NH4Cl, CsClon theactivityandstability ofthehorse liveralcoholdehydrogenase enzyme (HLADH) were investigated.Results: Itwasfoundthatboth enzymeactivityandstability oftheenzyme arerelatedto the (B−,B+) valuesofthe salts. In the presenceofsalt whoseanionsandcations have similar kosmotropic/chaotropic properties (NaAcandNaCl), catalytic activity of enzymeshowed thebestconditions. kosmotropic/chaotropicpropertiesofbothcationandanionaffect the catalytic activity ofthe enzyme by having an impact on the active siteand catalytic mechanismand surface pH.Anions comparedwith cationshad a more predominantrole inthestability oftheenzyme. Acetateanionmayinducegreaterthermal stability (half-life increasedfrom 300to 560minat 60oC).Conclusion: The presentworkhas demonstrated explicitlythe impact ofinorganic saltsandions on both the activityandstability ofhorse liver alcohol dehydrogenase. The stabilitystudyof alcohol dehydrogenaseoffersanotherexampleofmorekosmotropic anions and chaotropic cations favoring higher enzyme stability.

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APA: Copy

SHARIFI, RASOUL, SOLEIMANI, SALEH, AMINI, NAZILA, SAMIRA, SHARIFI, ATYABI, SEYED MOHAMMAD, & MIROLIAEI, MEHRAN. (2014). STABILITY AND ACTIVITY OF ALCOHOL DEHYDROGENASE IN THE PRESENCE OF KOSMOTROPE ANDCHAOTROPE SALT SERIES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 4(14), 73-78. SID. https://sid.ir/paper/204549/en

Vancouver: Copy

SHARIFI RASOUL, SOLEIMANI SALEH, AMINI NAZILA, SAMIRA SHARIFI, ATYABI SEYED MOHAMMAD, MIROLIAEI MEHRAN. STABILITY AND ACTIVITY OF ALCOHOL DEHYDROGENASE IN THE PRESENCE OF KOSMOTROPE ANDCHAOTROPE SALT SERIES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2014;4(14):73-78. Available from: https://sid.ir/paper/204549/en

IEEE: Copy

RASOUL SHARIFI, SALEH SOLEIMANI, NAZILA AMINI, SHARIFI SAMIRA, SEYED MOHAMMAD ATYABI, and MEHRAN MIROLIAEI, “STABILITY AND ACTIVITY OF ALCOHOL DEHYDROGENASE IN THE PRESENCE OF KOSMOTROPE ANDCHAOTROPE SALT SERIES,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 4, no. 14, pp. 73–78, 2014, [Online]. Available: https://sid.ir/paper/204549/en

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