Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium species

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BEIKI AMIR H.

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:30 Page(s): 95-110

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Title

Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium species

Author(s)

BEIKI AMIR H. | Issue Writer Certificate

Keywords

PhylogeneticQ1

AlignmentQ2

ITSQ1

Abstract

Aim and Background: Fusarium species are capable of infecting a wide range of crop plants. They can rarely cause human infections. Identification of Fusaria to the species level is necessary for biological, epidemiological, pathological, and toxicological purposes. The aim of this study was to evaluate Phylogenetic relationships and finding specified repetitive patterns clustered in the area of ITS in order to identify different species of Fusarium. Materials and Methods: Ribosomal DNA internal spacer region was amplified by polymerase chain reaction. ITS PCR product sequenced and analyzed by different methods. Results: Amplification resulted in approximately 550 bp in all the isolates. In the phylogram derived from analysis of the ITS region, the Fusarium spp. examined were divided into ten groups Discission: As expected the ITS region was variable and informative. The high level of variation between internal transcribed spacer is concluded from the elimination or addition of repeating unITS is in this area. Conclusion Some strains are identified and some isolates definitely has a different pattern. There are repetitive patterns at the ITS in the particular species suggesting that this motifs can be used as a tool to identify species.

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APA: Copy

BEIKI, AMIR H.. (2018). Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium species. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(30 ), 95-110. SID. https://sid.ir/paper/204558/en

Vancouver: Copy

BEIKI AMIR H.. Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium species. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(30 ):95-110. Available from: https://sid.ir/paper/204558/en

IEEE: Copy

AMIR H. BEIKI, “Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium species,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 30 , pp. 95–110, 2018, [Online]. Available: https://sid.ir/paper/204558/en

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