IDENTIFICATION AND GENOTYPIC CHARACTERIZATION OF NON-STARTER LACTIC ACID BACTERIA IN MATURE TALESHI CHEESE

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KAFILI TIVA; ALINESAII MOHSEN

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2017 | Volume:7 | Issue:26 Page(s): 111-117

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Title

IDENTIFICATION AND GENOTYPIC CHARACTERIZATION OF NON-STARTER LACTIC ACID BACTERIA IN MATURE TALESHI CHEESE

Author(s)

KAFILI TIVA | ALINESAII MOHSEN | Issue Writer Certificate

Keywords

LACTIC ACID BACTERIAQ1

RAPD-PCRQ1

TALESHI CHEESEQ1

TRADITIONAL CHEESEQ3

GENETIC DIVERSITYQ2

Abstract

Aim and Background: The development of molecular methods such as RAPD-PCR and 16Sr-RNA sequencing has facilitated the identification and evaluation of microbial diversity of complex microbial environment like fermented food. The aim of this study is identifying and genotyping of different LACTIC ACID BACTERIA genius and species involved in maturation of traditional TALESHI CHEESE.Methods: About 54 wild isolates on MRS and M17 mediums were selected and analyzed by culture dependent RAPD-PCR technique. Gel patterns derived by randomly designed M13 and D8635 primers clustered based on Pearson product moment correlation coefficient and UPGMA via MVSP software.16SrRNA sequencing has been used for species identification.Results: The results revealed dominate non-starter LACTIC ACID BACTERIA among isolates belonged to Lactobacillus genus including paraplantarum, curvatus, Sakei, paracase species following by Streptococcus gallolyticusand Enterococcus faecalis / Enterococcus faecium.Conclusion: The results showed that RAPD-PCR and 16SrRNA sequencing were useful and rapid tools for grouping, strain discrimination and identification of LACTIC ACID BACTERIA. Also, obtained 26 different genotypes represented a wide GENETIC DIVERSITY in this type of TRADITIONAL CHEESE.

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APA: Copy

KAFILI, TIVA, & ALINESAII, MOHSEN. (2017). IDENTIFICATION AND GENOTYPIC CHARACTERIZATION OF NON-STARTER LACTIC ACID BACTERIA IN MATURE TALESHI CHEESE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 7(26 ), 111-117. SID. https://sid.ir/paper/204613/en

Vancouver: Copy

KAFILI TIVA, ALINESAII MOHSEN. IDENTIFICATION AND GENOTYPIC CHARACTERIZATION OF NON-STARTER LACTIC ACID BACTERIA IN MATURE TALESHI CHEESE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2017;7(26 ):111-117. Available from: https://sid.ir/paper/204613/en

IEEE: Copy

TIVA KAFILI, and MOHSEN ALINESAII, “IDENTIFICATION AND GENOTYPIC CHARACTERIZATION OF NON-STARTER LACTIC ACID BACTERIA IN MATURE TALESHI CHEESE,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 7, no. 26 , pp. 111–117, 2017, [Online]. Available: https://sid.ir/paper/204613/en

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