FREQUENCY OF SINGLE NUCLEOTIDE POLYMORPHISM OF TNF--α GENE (RS1800629) IN IRANIAN PATIENTS WITH HEPATITIS

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BAHIRAEI NARGES; SADAT SEYED MEHDI; Baghbani Arani Fahimeh

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2017 | Volume:7 | Issue:26 Page(s): 119-128

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Title

FREQUENCY OF SINGLE NUCLEOTIDE POLYMORPHISM OF TNF--α GENE (RS1800629) IN IRANIAN PATIENTS WITH HEPATITIS

Author(s)

BAHIRAEI NARGES | SADAT SEYED MEHDI | Baghbani Arani Fahimeh | Issue Writer Certificate

Keywords

HEPATITIS CQ2

TUMOR NECROSIS FACTOR-ALPHA (TNF-α)Q1

POLYMORPHISMQ2

RS1800629Q2

IRANQ1

Abstract

Aim and Background: Recent studies have claimed that the genetic variation in cytokine production systems has a major effect on immune system potency and strongly associated with the antiviral treatment response. On the other hand, tumor necrosis factor-alpha (TNF-a), a multifunctional pro-inflammatory cytokine, plays an essential role in host immune response to HCV infection. Therefore, current study was done in order to determine the distribution of the RS1800629 (A/G) POLYMORPHISM in the promoter of TNF-a gene in IRANian population.Material and methods: This cross-sectional study, performed on 165 blood samples based on 89 HCV infected patients including 68 SVR positive and 21 negative and 76 healthy individual controls. After DNA extraction the frequency of RS1800629 (A/G) POLYMORPHISM was analyzed using PCRRFLP method. For detection and analysis of the PCR products we used 20% Polyacrylamide gel electrophoresis.Results: Based on analysis of the data, the distribution of the A/G POLYMORPHISM between healthy individuals and patients were obtained as AA: 1.1%, AG: 52.6%, GG: 46.1%, and AA: 1.3%, AG: 20.2%, GG: 78.7% respectively. The GG genotype was identified in 70 patients of whom 55 achieved SVR, while the AG heterozygous was found in 18 patients and SVR was achieved in 12. Finally, the AA was detected only in one patient with positive SVR.Conclusion: In this study, significant difference between the TNF-a-308 locus SNP (rs1800629) and susceptibility to chronic HCV infection in IRANian population (P value=0.002) was observed. Moreover, the presence of G allele among SVR positive people and non-SVR group did not show strong statistic difference (P value=0.476). However, further studies with more samples seems to be necessary.

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APA: Copy

BAHIRAEI, NARGES, SADAT, SEYED MEHDI, & Baghbani Arani, Fahimeh. (2017). FREQUENCY OF SINGLE NUCLEOTIDE POLYMORPHISM OF TNF--α GENE (RS1800629) IN IRANIAN PATIENTS WITH HEPATITIS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 7(26 ), 119-128. SID. https://sid.ir/paper/204614/en

Vancouver: Copy

BAHIRAEI NARGES, SADAT SEYED MEHDI, Baghbani Arani Fahimeh. FREQUENCY OF SINGLE NUCLEOTIDE POLYMORPHISM OF TNF--α GENE (RS1800629) IN IRANIAN PATIENTS WITH HEPATITIS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2017;7(26 ):119-128. Available from: https://sid.ir/paper/204614/en

IEEE: Copy

NARGES BAHIRAEI, SEYED MEHDI SADAT, and Fahimeh Baghbani Arani, “FREQUENCY OF SINGLE NUCLEOTIDE POLYMORPHISM OF TNF--α GENE (RS1800629) IN IRANIAN PATIENTS WITH HEPATITIS,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 7, no. 26 , pp. 119–128, 2017, [Online]. Available: https://sid.ir/paper/204614/en

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