CLONING HEPATITIS C VIRUS E2 GENE FULL-LENGTH FROM GENOTYPE 2A (JFH1) TO PET28A (+) VECTOR AND TRANSFORMATION OF AN ESCHERICHIA COLI DH5α STRAIN

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BAGHERI SARA; RAHIMI POONEH; VAHABPOUR ROUHOLAH; SADAT SEYED MEHDI; AGHASADEGHI MOHAMMAD REZA; MOTEVALLI FATEMEH

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2016 | Volume:6 | Issue:22 Page(s): 17-22

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Title

CLONING HEPATITIS C VIRUS E2 GENE FULL-LENGTH FROM GENOTYPE 2A (JFH1) TO PET28A (+) VECTOR AND TRANSFORMATION OF AN ESCHERICHIA COLI DH5α STRAIN

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Keywords

HEPATITIS C VIRUSQ1

E2 GENE FULL-LENGTHQ1

GENOTYPE 2A (JFH1)Q1

PET28A (+) VECTORQ1

E-COLI (DH5α)Q1

Abstract

Aim and Background: The envelope glycoprotein E2 in HEPATITIS C VIRUS is required for host-cell entry. The nucleotide sequence diversity in carboxyl-terminus (C-terminal) of this gene results in induction of neutralizing antibody that makes this gene an important target for vaccine development studies. In this study we tried to make a recombinant pET28a (+) containing full length of the E2 gene from genotype 2a (JFH1) followed by being transformed into the E-coli (DH5a).Material and Methods: Full-length of HCV E2 region from genotype 2a (JFH1) was amplified by PCR reaction using the specific primers including the restriction sites for EcoR1 and Nde1. The confirmed PCR product was cloned into the pET28a (+) vector and transformed into the E-coli (DH5a) using the heat shock method.Results: The HCV E2 gene was successfully cloned into the pET28a (+) vector in full length size. Transformation of the E-coli strain DH5a by this recombinant vector was confirmed.Conclusion: Transformation of the E-coli (DH5a) using the recombinant vector pET28a- HCV 2a (JFH1) E2 gene provides useful tools for further expression of this gene in full length size in different expression system.

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APA: Copy

BAGHERI, SARA, RAHIMI, POONEH, VAHABPOUR, ROUHOLAH, SADAT, SEYED MEHDI, AGHASADEGHI, MOHAMMAD REZA, & MOTEVALLI, FATEMEH. (2016). CLONING HEPATITIS C VIRUS E2 GENE FULL-LENGTH FROM GENOTYPE 2A (JFH1) TO PET28A (+) VECTOR AND TRANSFORMATION OF AN ESCHERICHIA COLI DH5α STRAIN. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 6(22), 17-22. SID. https://sid.ir/paper/204624/en

Vancouver: Copy

BAGHERI SARA, RAHIMI POONEH, VAHABPOUR ROUHOLAH, SADAT SEYED MEHDI, AGHASADEGHI MOHAMMAD REZA, MOTEVALLI FATEMEH. CLONING HEPATITIS C VIRUS E2 GENE FULL-LENGTH FROM GENOTYPE 2A (JFH1) TO PET28A (+) VECTOR AND TRANSFORMATION OF AN ESCHERICHIA COLI DH5α STRAIN. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2016;6(22):17-22. Available from: https://sid.ir/paper/204624/en

IEEE: Copy

SARA BAGHERI, POONEH RAHIMI, ROUHOLAH VAHABPOUR, SEYED MEHDI SADAT, MOHAMMAD REZA AGHASADEGHI, and FATEMEH MOTEVALLI, “CLONING HEPATITIS C VIRUS E2 GENE FULL-LENGTH FROM GENOTYPE 2A (JFH1) TO PET28A (+) VECTOR AND TRANSFORMATION OF AN ESCHERICHIA COLI DH5α STRAIN,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 6, no. 22, pp. 17–22, 2016, [Online]. Available: https://sid.ir/paper/204624/en

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