STUDY OF THE GENOMES EXPRESSED IN THE CELLS OF THE LEAF TRICHOMES FOR DETERMINING OF IMPORTANT GENOMIC ELEMENTS IN MEDICINAL HERB; SALVIA FRUTICOSA

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FOOLADVAND ZIBA; FAZELI NASAB BAHMAN

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2016 | Volume:6 | Issue:23 Page(s): 77-85

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Title

STUDY OF THE GENOMES EXPRESSED IN THE CELLS OF THE LEAF TRICHOMES FOR DETERMINING OF IMPORTANT GENOMIC ELEMENTS IN MEDICINAL HERB; SALVIA FRUTICOSA

Author(s)

FOOLADVAND ZIBA | FAZELI NASAB BAHMAN | Issue Writer Certificate

Keywords

FUNCTIONAL GENOMICQ1

TRICHOME LEAFQ1

MEDICINAL HERBQ1

Abstract

Aim & Background: Strategy genomics such as analysis of EST sequence, provide to identify markers associated with gene expressed and miRNA related regulatory and metabolic networks. In this study identifying elements associated with genome expressed were examined in Salvia fruticosa leaf trichomes. Material and methods: After collecting and removal of vector sequences, chloroplasts, repetitive, assembly them, were created Singletons and contigs sequences. Using by the BlastX, current hits and frequency and distribution of EST-SSR markers were determined using SSRTools. Related miRNA target sequence using modeling and search were identified in Arabidopsis thaliana.Results: 153 contigs and 777 Singletons was found. BLASTX has revealed 477 unigene with hits. Gene enrichment analysis sequences in different functional groups of the contract. The molecular markers EST-SSRs identified di-nucleotide AT / GA, tri- nucleotides GCC, Tetra- nucleotides TAAT repeated at least four most frequent and penta and hexa-nucleotide repeat of AAGAG and AGTATT, CGTGGT three tandem repeats a times reported. miRNA gene expression were identified related to a number of 40 different genes.Conclusion: EST-SSRs identified in the plant can be used to determine the variation between different species of this genus in the plant family lamiaceae. Two related miRNA (monoterpene synthase) to regulate the expression of genes important in producing terpenoids important to be used in the plant.

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APA: Copy

FOOLADVAND, ZIBA, & FAZELI NASAB, BAHMAN. (2016). STUDY OF THE GENOMES EXPRESSED IN THE CELLS OF THE LEAF TRICHOMES FOR DETERMINING OF IMPORTANT GENOMIC ELEMENTS IN MEDICINAL HERB; SALVIA FRUTICOSA. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 6(23), 77-85. SID. https://sid.ir/paper/204725/en

Vancouver: Copy

FOOLADVAND ZIBA, FAZELI NASAB BAHMAN. STUDY OF THE GENOMES EXPRESSED IN THE CELLS OF THE LEAF TRICHOMES FOR DETERMINING OF IMPORTANT GENOMIC ELEMENTS IN MEDICINAL HERB; SALVIA FRUTICOSA. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2016;6(23):77-85. Available from: https://sid.ir/paper/204725/en

IEEE: Copy

ZIBA FOOLADVAND, and BAHMAN FAZELI NASAB, “STUDY OF THE GENOMES EXPRESSED IN THE CELLS OF THE LEAF TRICHOMES FOR DETERMINING OF IMPORTANT GENOMIC ELEMENTS IN MEDICINAL HERB; SALVIA FRUTICOSA,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 6, no. 23, pp. 77–85, 2016, [Online]. Available: https://sid.ir/paper/204725/en

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