DETECTION AND CHARACTERIZATION OF 30 KDA HEAT SHOCK PROTEIN IN DERMATOPHYTE FUNGUS OF TRICHOPHYTON RUBRUM

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BEHZADI E.; FALAHIAN F.A.; BEBZADI P.; REZAEI SASAN

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Journal: JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT) Year:2007 | Volume:32 | Issue:4 (SECTION: BIOLOGY 2) Page(s): 327-333

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Title

DETECTION AND CHARACTERIZATION OF 30 KDA HEAT SHOCK PROTEIN IN DERMATOPHYTE FUNGUS OF TRICHOPHYTON RUBRUM

Author(s)

BEHZADI E. | FALAHIAN F.A. | BEBZADI P. | REZAEI SASAN | Issue Writer Certificate

Keywords

TRHSP30Q3

TRICHOPHYTON RUBRUMQ3

PCRQ3

Abstract

Heat shock protein 30 (HSP30) genes are conserved in their structural properties and are widely spread.HSP70 in TRICHOPHYTON RUBRUM is already detected and carefully characterized; however, no study was carried out for HSP70 in this pathogen fungus. In this survey, T. rubrum was obtained from patients with dermatophytosis and cultured in appropriate condition. High Molecular weight DNA has been extracted by using standard extraction methods.Pairs of 21 nt primers were designed from highly conserved regions of the same gene in other eukaryotic cells. Mentioned primers were utilized in PCR fragments were then sequenced. Finally, 415 nucleotides of HSP70 in this pathogen fungus were detected; which its ORF had 156 nucleotides and was coding 51 amino acids. This gene (called TRHSP30) is registered in Genbank at NCBI (NIH, USA) database. Detection of this gene may open the way to determination of its possible role in the pathogenesis of dermatophyte infections due to T. rubrum.

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References

THE EFFECTS OF UVB-IRRADIATION ON DERMATOPHYTE FUNGI

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APA: Copy

BEHZADI, E., FALAHIAN, F.A., BEBZADI, P., & REZAEI, SASAN. (2007). DETECTION AND CHARACTERIZATION OF 30 KDA HEAT SHOCK PROTEIN IN DERMATOPHYTE FUNGUS OF TRICHOPHYTON RUBRUM. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), 32(4 (SECTION: BIOLOGY 2)), 327-333. SID. https://sid.ir/paper/2075/en

Vancouver: Copy

BEHZADI E., FALAHIAN F.A., BEBZADI P., REZAEI SASAN. DETECTION AND CHARACTERIZATION OF 30 KDA HEAT SHOCK PROTEIN IN DERMATOPHYTE FUNGUS OF TRICHOPHYTON RUBRUM. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT)[Internet]. 2007;32(4 (SECTION: BIOLOGY 2)):327-333. Available from: https://sid.ir/paper/2075/en

IEEE: Copy

E. BEHZADI, F.A. FALAHIAN, P. BEBZADI, and SASAN REZAEI, “DETECTION AND CHARACTERIZATION OF 30 KDA HEAT SHOCK PROTEIN IN DERMATOPHYTE FUNGUS OF TRICHOPHYTON RUBRUM,” JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), vol. 32, no. 4 (SECTION: BIOLOGY 2), pp. 327–333, 2007, [Online]. Available: https://sid.ir/paper/2075/en

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