PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23)

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ALANI B.; MOTALEBI M.; ZAMANI M.R.

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ISSN: 2588-4824

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2004 | Volume:16 | Issue:4 Page(s): 1-12

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Title

PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23)

Author(s)

ALANI B. | MOTALEBI M. | ZAMANI M.R. | Issue Writer Certificate

Keywords

PLOYGLOACTORONSEQ4

FUSRIUM OXYSPORUMQ4

CLICK-PEA DISEASEQ4

Abstract

Fusarium wilt caused by Fusarium oxysporum is an important problem in Chick-pea (Cicer arietinum). This disease is one of the most effective factors of Chick-pea crop losses in the West and North-west of Iran. Fusarium oxysporum produces a wide range of enzymes including polygalacturonase capable for degradation of the structural components of the plant cell wall.Purification of polygalacturonase enzymes from isolate F23 of Fusarium oxysporum was achieved by cation exchange chromatography on CM-Sepharose Fast Flow column equilibrated to pH 5.5 with 50mM sodium acetate buffer. Elution of the column was carried out with 1M NaCl gradient. The presence of PG enzymes was investigated by SDS-PAGE. The results showed two distinct bands, about 38 and 40 kDa in isolate F23. These two bands showed PG activity in gel containing pectin as substrate (Zymogram). The optimum pH for enzyme activity before and after purification was determined to be about 4.5

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APA: Copy

ALANI, B., MOTALEBI, M., & ZAMANI, M.R.. (2004). PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23). IRANIAN JOURNAL OF BIOLOGY, 16(4), 1-12. SID. https://sid.ir/paper/21013/en

Vancouver: Copy

ALANI B., MOTALEBI M., ZAMANI M.R.. PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23). IRANIAN JOURNAL OF BIOLOGY[Internet]. 2004;16(4):1-12. Available from: https://sid.ir/paper/21013/en

IEEE: Copy

B. ALANI, M. MOTALEBI, and M.R. ZAMANI, “PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23),” IRANIAN JOURNAL OF BIOLOGY, vol. 16, no. 4, pp. 1–12, 2004, [Online]. Available: https://sid.ir/paper/21013/en

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