PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ALANI B.; MOTALEBI M.; ZAMANI M.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2004 | Volume:16 | Issue:4 Page(s): 1-12

Download Full-Text

Persian Verion

View:

1,038

Download:

Cites:

Information Journal Paper

Title

PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23)

Author(s)

ALANI B. | MOTALEBI M. | ZAMANI M.R. | Issue Writer Certificate

Keywords

PLOYGLOACTORONSEQ4

FUSRIUM OXYSPORUMQ4

CLICK-PEA DISEASEQ4

Abstract

Fusarium wilt caused by Fusarium oxysporum is an important problem in Chick-pea (Cicer arietinum). This disease is one of the most effective factors of Chick-pea crop losses in the West and North-west of Iran. Fusarium oxysporum produces a wide range of enzymes including polygalacturonase capable for degradation of the structural components of the plant cell wall.Purification of polygalacturonase enzymes from isolate F23 of Fusarium oxysporum was achieved by cation exchange chromatography on CM-Sepharose Fast Flow column equilibrated to pH 5.5 with 50mM sodium acetate buffer. Elution of the column was carried out with 1M NaCl gradient. The presence of PG enzymes was investigated by SDS-PAGE. The results showed two distinct bands, about 38 and 40 kDa in isolate F23. These two bands showed PG activity in gel containing pectin as substrate (Zymogram). The optimum pH for enzyme activity before and after purification was determined to be about 4.5

Cites

No record.

References

No record.

Cite

APA: Copy

ALANI, B., MOTALEBI, M., & ZAMANI, M.R.. (2004). PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23). IRANIAN JOURNAL OF BIOLOGY, 16(4), 1-12. SID. https://sid.ir/paper/21013/en

Vancouver: Copy

ALANI B., MOTALEBI M., ZAMANI M.R.. PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23). IRANIAN JOURNAL OF BIOLOGY[Internet]. 2004;16(4):1-12. Available from: https://sid.ir/paper/21013/en

IEEE: Copy

B. ALANI, M. MOTALEBI, and M.R. ZAMANI, “PURIFICATION AND PARTIAL CHARACTERIZATION OF POLYGALACTURONASE FROM HIGHLY VIRULENT (HV) ISOLATE OF FUSARIUM OXYSPORUM (F23),” IRANIAN JOURNAL OF BIOLOGY, vol. 16, no. 4, pp. 1–12, 2004, [Online]. Available: https://sid.ir/paper/21013/en

Related Journal Papers