DIRECT DNA IMMOBILIZATION ONTO A CARBON NANOTUBE MODIFIED ELECTRODE: STUDY ON THE INFLUENCE OF PH AND IONIC STRENGTH

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RAFIEE POUR HOSSAIN ALI; BEHPOUR MOHSEN; KESHAVARZ MAHIN

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Journal: JOURNAL OF NANOSTRUCTURES Year:2016 | Volume:6 | Issue:3 Page(s): 235-244

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Title

DIRECT DNA IMMOBILIZATION ONTO A CARBON NANOTUBE MODIFIED ELECTRODE: STUDY ON THE INFLUENCE OF PH AND IONIC STRENGTH

Author(s)

RAFIEE POUR HOSSAIN ALI | BEHPOUR MOHSEN | KESHAVARZ MAHIN | Issue Writer Certificate

Keywords

DNA IMMOBILIZATIONQ2

CARBOXYLIC ACID-FUNCTIONALIZEDQ2

CARBON NANOTUBESQ2

COVALENT INTERACTIONQ2

STANDARD FREE-ENERGY OFQ2

ADSORPTIONQ2

Abstract

Over the past years, DNA biosensors have been developed to analyze DNA interaction and damage that have important applications in biotechnological researches. The immobilization of DNA onto a substrate is one key step for construction of DNA electrochemical biosensors. In this report, a direct approach has been described for immobilization of single strand DNA onto CARBOXYLIC ACID-FUNCTIONALIZED CARBON NANOTUBES modified glassy carbon electrode. To do this, we first modified the glassy carbon electrode surface with MWCNT-COOH. The immersion of MWCNT-COOH/ GCE in ss-DNA probe solution, with different pH and ionic strength, was followed by suitable interaction between amine group of ss-DNA bases and carboxylic groups of MWCNT-COOH. This interaction leads to successful ss-DNA IMMOBILIZATION on MWCNT-COOH that was confirmed by cyclic voltammetry, electrochemical impedance spectroscopy and atomic force microscopy. Immobilization of ss-DNA on the modified electrode increased the charge transfer resistant but decreased the peak current of redox probe ([Fe (CN)6] 3-/4-). The result of cyclic voltammograms implicates that enhancements in the DNA IMMOBILIZATION are possible by adroit choice of low pH and high ionic strength. The STANDARD FREE-ENERGY OF ADSORPTION ( DGoads) was calculated from electrochemical impedance spectroscopy data (-47.75 kJ mol-1) and was confirmed covalent bond formation. atomic force microscopy topographic images demonstrate increased surface roughness after ss-DNA IMMOBILIZATION. Results offer a simple, rapid and low-cost of DNA IMMOBILIZATION strategy can be opportunities to design of novel nucleic acid biosensors.

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APA: Copy

RAFIEE POUR, HOSSAIN ALI, BEHPOUR, MOHSEN, & KESHAVARZ, MAHIN. (2016). DIRECT DNA IMMOBILIZATION ONTO A CARBON NANOTUBE MODIFIED ELECTRODE: STUDY ON THE INFLUENCE OF PH AND IONIC STRENGTH. JOURNAL OF NANOSTRUCTURES, 6(3), 235-244. SID. https://sid.ir/paper/210871/en

Vancouver: Copy

RAFIEE POUR HOSSAIN ALI, BEHPOUR MOHSEN, KESHAVARZ MAHIN. DIRECT DNA IMMOBILIZATION ONTO A CARBON NANOTUBE MODIFIED ELECTRODE: STUDY ON THE INFLUENCE OF PH AND IONIC STRENGTH. JOURNAL OF NANOSTRUCTURES[Internet]. 2016;6(3):235-244. Available from: https://sid.ir/paper/210871/en

IEEE: Copy

HOSSAIN ALI RAFIEE POUR, MOHSEN BEHPOUR, and MAHIN KESHAVARZ, “DIRECT DNA IMMOBILIZATION ONTO A CARBON NANOTUBE MODIFIED ELECTRODE: STUDY ON THE INFLUENCE OF PH AND IONIC STRENGTH,” JOURNAL OF NANOSTRUCTURES, vol. 6, no. 3, pp. 235–244, 2016, [Online]. Available: https://sid.ir/paper/210871/en

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