EFFICIENCY OF ULTRA VIOLET AND ETHIDIUM BROMIDE TREATMENTS IN INCREASING OF RESISTANCE TO ERYTHROMYCIN IN SACCHAROPOLYSPORA ERYTHRAEA MUTANTS

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HAMEDI J.; KHODAGHOLI F.; AMOUZGAR M.A.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2003 | Volume:13 | Issue:3-4 Page(s): 8-17

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Title

EFFICIENCY OF ULTRA VIOLET AND ETHIDIUM BROMIDE TREATMENTS IN INCREASING OF RESISTANCE TO ERYTHROMYCIN IN SACCHAROPOLYSPORA ERYTHRAEA MUTANTS

Author(s)

HAMEDI J. | KHODAGHOLI F. | AMOUZGAR M.A. | Issue Writer Certificate

Keywords

ANTIBIOTIC RESISTANCEQ2

ERYTHROMYCINQ4

SACCHAROPOLYSPORA ERYTHRAEAQ3

UV RADIATIONQ2

ETHIDIUM BROMIDEQ2

MUTAGENESISQ4

Abstract

Mutation has been used as an efficient method for improving the suitable characteristics in industrial microorganisms. In this research we compared the effect of UV RADIATION, the most important physical agent and treatment with ETHIDIUM BROMIDE on MUTAGENESIS of SACCHAROPOLYSPORA ERYTHRAEA NUR001, the producer of ERYTHROMYCIN. The spores of S. erythraea NUR001 was exposed to the various doses of the UV RADIATION and various concentrations of ETHIDIUM BROMIDE, and then screening method was used for the isolation of the mutants resistant to ERYTHROMYCIN. The results obtained revealed that optimum conditions for MUTAGENESIS with UV were 5cm distance from a 254nm UV source and 45 minutes radiation. The best concentration of ETHIDIUM BROMIDE for MUTAGENESIS was 10µg/ml. The results showed that UV RADIATION was more efficient than ETHIDIUM BROMIDE in the production of mutants that showing increased resistance to ERYTHROMYCIN. The mutant exhibited higher resistance (9µg/ml) to ERYTHROMYCIN when compared with that of the parent strain (8Ilg/ml). In this research, the beneficial MUTAGENESIS effect of UV on SACCHAROPOLYSPORA ERYTHRAEA NUR001 was more than ETHIDIUM BROMIDE. We obtained the SACCHAROPOLYSPORA ERYTHRAEA NUR002.Compared to its parents, this strain was more resistance to high concentration of ERYTHROMYCIN.

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APA: Copy

HAMEDI, J., KHODAGHOLI, F., & AMOUZGAR, M.A.. (2003). EFFICIENCY OF ULTRA VIOLET AND ETHIDIUM BROMIDE TREATMENTS IN INCREASING OF RESISTANCE TO ERYTHROMYCIN IN SACCHAROPOLYSPORA ERYTHRAEA MUTANTS. IRANIAN JOURNAL OF BIOLOGY, 13(3-4), 8-17. SID. https://sid.ir/paper/21214/en

Vancouver: Copy

HAMEDI J., KHODAGHOLI F., AMOUZGAR M.A.. EFFICIENCY OF ULTRA VIOLET AND ETHIDIUM BROMIDE TREATMENTS IN INCREASING OF RESISTANCE TO ERYTHROMYCIN IN SACCHAROPOLYSPORA ERYTHRAEA MUTANTS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2003;13(3-4):8-17. Available from: https://sid.ir/paper/21214/en

IEEE: Copy

J. HAMEDI, F. KHODAGHOLI, and M.A. AMOUZGAR, “EFFICIENCY OF ULTRA VIOLET AND ETHIDIUM BROMIDE TREATMENTS IN INCREASING OF RESISTANCE TO ERYTHROMYCIN IN SACCHAROPOLYSPORA ERYTHRAEA MUTANTS,” IRANIAN JOURNAL OF BIOLOGY, vol. 13, no. 3-4, pp. 8–17, 2003, [Online]. Available: https://sid.ir/paper/21214/en

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