STUDY OF GLACTOSE IN THE EXTRACTED SOLUBLE SUGARS FROM THREE EUCALYPTUS SPECIES EXPLANTS AS A MARKER OF EXPLANTS GROWTH CONDITIONS

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EBRAHIMZADEH H.; ABBASI MOHAMMAD REZA; FAKHR TABATABAEI S.M.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2006 | Volume:18 | Issue:4 Page(s): 280-294

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Title

STUDY OF GLACTOSE IN THE EXTRACTED SOLUBLE SUGARS FROM THREE EUCALYPTUS SPECIES EXPLANTS AS A MARKER OF EXPLANTS GROWTH CONDITIONS

Author(s)

EBRAHIMZADEH H. | ABBASI MOHAMMAD REZA | FAKHR TABATABAEI S.M. | Issue Writer Certificate

Keywords

EUCALYPTUSQ2

GALACTOSEQ4

TISSUE CULTUERQ2

Abstract

Hypocotyledon explants of EUCALYPTUS camaldulensis Dehnh., E. froticetorum E. Muell and E. viminalis Labill were cultured on LS medium with three hormonal treatments at 24 0C and were used to produce callus and root. The non-structural carbohydrates were extracted. These sugars were quantitatively and qualitatively analyzed based on spectrophotometry, thin layer chromatography and gas liquid chromatography. A hormonal treatment with a combination of 0.88 µM BA, 2.6 µM NAA, 2.8 µM IBA, and 0.9 µM 2, 4-D which produced the dark woody callus, induced the highest growth rate. Whereas in 20 µM 2,4-D the light-fresh callus were produced. At IBA 10 µM the explants produced too much root. The major sugars in the nonhydorized of soluble sugars extraction were sucrose and glucose and a little amount of romnose, fructose, ribose and glucoronic acid. Also in this fraction there was no any GALACTOSE. In the its hydrolyzed fraction there was no GALACTOSE, whereas glucose and fructose which was produced from sucrose were the major sugars in this fraction. In ydrolyzed nonstructural - polysaccharide fractions, GALACTOSE, glucose, mannose – arabinose, xylose and one uronic acid were appeared in all treatments and species. Contrary to sugars in intact plants of EUCALYPTUS tree, there was no any GALACTOSE in the hydrolyzed soluble sugars fraction in the tissue culture extractions, but it was appeared in the hydrolyzed nonstructural - polysaccharides fraction of explant extractions. The absence of GALACTOSE in the hydrolyzed or unhydrolized soluble extraction sugars could serve as a marker or an indicator for optimum conditions of growth of explants.

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APA: Copy

EBRAHIMZADEH, H., ABBASI, MOHAMMAD REZA, & FAKHR TABATABAEI, S.M.. (2006). STUDY OF GLACTOSE IN THE EXTRACTED SOLUBLE SUGARS FROM THREE EUCALYPTUS SPECIES EXPLANTS AS A MARKER OF EXPLANTS GROWTH CONDITIONS . IRANIAN JOURNAL OF BIOLOGY, 18(4), 280-294. SID. https://sid.ir/paper/21250/en

Vancouver: Copy

EBRAHIMZADEH H., ABBASI MOHAMMAD REZA, FAKHR TABATABAEI S.M.. STUDY OF GLACTOSE IN THE EXTRACTED SOLUBLE SUGARS FROM THREE EUCALYPTUS SPECIES EXPLANTS AS A MARKER OF EXPLANTS GROWTH CONDITIONS . IRANIAN JOURNAL OF BIOLOGY[Internet]. 2006;18(4):280-294. Available from: https://sid.ir/paper/21250/en

IEEE: Copy

H. EBRAHIMZADEH, MOHAMMAD REZA ABBASI, and S.M. FAKHR TABATABAEI, “STUDY OF GLACTOSE IN THE EXTRACTED SOLUBLE SUGARS FROM THREE EUCALYPTUS SPECIES EXPLANTS AS A MARKER OF EXPLANTS GROWTH CONDITIONS ,” IRANIAN JOURNAL OF BIOLOGY, vol. 18, no. 4, pp. 280–294, 2006, [Online]. Available: https://sid.ir/paper/21250/en

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