CLONING OF NEW FORM OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (K2S) IN EUKARYOTIC SYSTEM

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NAZARI R.; DAVOUDI N.; BARKHORDARI F.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2011 | Volume:24 | Issue:5 Page(s): 632-639

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Title

CLONING OF NEW FORM OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (K2S) IN EUKARYOTIC SYSTEM

Author(s)

NAZARI R. | DAVOUDI N. | BARKHORDARI F. | Issue Writer Certificate

Keywords

TPAQ2

RETEPLASEQ2

L.TARENTOLAEQ2

Abstract

Tissue plasminogen activator (TPA) protein was found as superior thrombolytic agent for treatment of diseases such as acute myocardial infarction and efforts is currently focused to improve the TPA molecule and thereby it's pharmacokinetic properties.Reteplase (K2S) is a derivative TPA that has a longer half-life and greater resistance to inhibitor than the natural TPA molecule. The aim of this research is cloning and expression of K2S form of the TPA cDNA in eukaryotic systemL. tarentolae which is recently has been introduced as a suitable host for expression eukaryotic genes. K2S form of TPA cDNA amplified by PCR and has cloned at ssurRNA (18s) site of L. tarentolaegenome by homologous recombination. Diagnostic PCR analysis showed integration of K2S expression cassette in 18srRNA gene. Performance of zymography analysis on culture supernatant of transfected L. tarentolae showed serine protease activity of the rK2S protein produced and demonstrate that rK2S protein produced in L. tarentolaecells was biologically active. Hence, L. tarentolae is the first useful biotechnologically eukaryotic organism and simple nutrient requirements; we hope to reduce the production cost of this protein by cloning of K2S gene in this system.

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APA: Copy

NAZARI, R., DAVOUDI, N., & BARKHORDARI, F.. (2011). CLONING OF NEW FORM OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (K2S) IN EUKARYOTIC SYSTEM. IRANIAN JOURNAL OF BIOLOGY, 24(5), 632-639. SID. https://sid.ir/paper/21300/en

Vancouver: Copy

NAZARI R., DAVOUDI N., BARKHORDARI F.. CLONING OF NEW FORM OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (K2S) IN EUKARYOTIC SYSTEM. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;24(5):632-639. Available from: https://sid.ir/paper/21300/en

IEEE: Copy

R. NAZARI, N. DAVOUDI, and F. BARKHORDARI, “CLONING OF NEW FORM OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (K2S) IN EUKARYOTIC SYSTEM,” IRANIAN JOURNAL OF BIOLOGY, vol. 24, no. 5, pp. 632–639, 2011, [Online]. Available: https://sid.ir/paper/21300/en

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