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Information Journal Paper

Title

UTILIZING SEVERAL GROWTH INHIBITORS FOR FINDING GIBBERELLIN SYNTHESIZING AFFECTING MUTANTS IN ARABIDOPSIS THALIANA

Pages

  778-788

Abstract

 Scientists have made much effort to identify MUTANTS which can help them to recognize GIBBERELLIN synthesizing pathway in plants. Therefore, effects of different concentrations of three chemical agents named UNICONAZOL, PRONAMIDE AND ISOXABEN on ARABIDOPSIS THALIANA growth was investigated, using existing variable Arabidopsis gene pools, to find MUTANTS by which inhibitory effects of the mentioned chemicals can be prevented.Critical concentration of the chemicals on seed germination and vegetative growth of Arabidopsis was first determined. Interaction effects of the levels close to critical concentration of the chemicals by which the MUTANTS may be identified were recognized.Combination effects of UNICONAZOL, Isoxaben and UNICONAZOL, Pronamide on seed germination and vegetative growth of Arabidopsis were investigated by two separate experiments. The combinations with the higher interaction effects were specified to be used by screening of several genetically variable gene pools of ARABIDOPSIS THALIANA.Significant interaction effects on vegetative growth of the Arabidopsis species were observed between several levels of the chemicals. At the highest applied level of UNICONAZOL (395 nM) the applied levels of Isoxaben and Pronamide showed more effects on roots than on hypocotyls growth. Pronamide effects on the reduction of hypocotyl and root growth were much more than those of Isoxaben. The chemical combinations effects showed reverse effects on root tip meristems. Although by increasing the chemicals concentration, hypocotyl and root length were decreased, increasing the concentrations, root tip meristem thicknesses were also increased. The variable Arabidopsis gene pools were sown on media containing the combinations of the chemicals concentration. A number of seeds were germinated and grown on the media. These were grown to maturity and seed production. Seeds were collected on the grown plants to be employed as MUTANTS RESISTANT to inhibitory effects of the chemicals in the future.

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    APA: Copy

    MIRZAIE NODOUSHAN, H.. (2011). UTILIZING SEVERAL GROWTH INHIBITORS FOR FINDING GIBBERELLIN SYNTHESIZING AFFECTING MUTANTS IN ARABIDOPSIS THALIANA. IRANIAN JOURNAL OF BIOLOGY, 24(5), 778-788. SID. https://sid.ir/paper/21301/en

    Vancouver: Copy

    MIRZAIE NODOUSHAN H.. UTILIZING SEVERAL GROWTH INHIBITORS FOR FINDING GIBBERELLIN SYNTHESIZING AFFECTING MUTANTS IN ARABIDOPSIS THALIANA. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;24(5):778-788. Available from: https://sid.ir/paper/21301/en

    IEEE: Copy

    H. MIRZAIE NODOUSHAN, “UTILIZING SEVERAL GROWTH INHIBITORS FOR FINDING GIBBERELLIN SYNTHESIZING AFFECTING MUTANTS IN ARABIDOPSIS THALIANA,” IRANIAN JOURNAL OF BIOLOGY, vol. 24, no. 5, pp. 778–788, 2011, [Online]. Available: https://sid.ir/paper/21301/en

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