THE EFFECT OF DIFFERENT CONCENTRATIONS OF β- GLUCAN ON INTRACELLULAR PROLACTIN SYNTHESIS IN GH3/B6 CELLS AND ITS RECEPTOR, DECTIN-1

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SHAERZADEH F.; SEPEHRI H.; HOSSEIN GH.; GOLIAEI B.; DELPHI L.; RASSOULI Y.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2011 | Volume:23 | Issue:5 Page(s): 663-671

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Title

THE EFFECT OF DIFFERENT CONCENTRATIONS OF β- GLUCAN ON INTRACELLULAR PROLACTIN SYNTHESIS IN GH3/B6 CELLS AND ITS RECEPTOR, DECTIN-1

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Keywords

GH3/B6 CELLSQ3

PROLACTINQ3

β-GLUCANQ3

DECTIN-1Q3

Abstract

Prolactin is a polypeptide hormone of the anterior pituitary gland that was originally named for its ability to promote lactation. Today we know that it has over 300 different function in vertebrates including osmoregulation, growth and development, metabolism, behavior, reproduction and immunoregulation. Recently, the role of PROLACTIN in immune system is investigated. Glucans are glucose polymers that are major constituents of the cell wall of fungi, plant and certain bacteria. In the present study, we used GH3/B6 cell line which is known to secrete PROLACTIN and growth hormone. We also studied the expression of b-glucan receptor, DECTIN-1, on this cells. Intracellular PROLACTIN levels were assayed by ELISA. Cells were treated with 5,150 and 250mgr/ml concentrations of b-glucan for incubation time of 48 hours. TRH (50 nM) was used as positive control and dextran (50nM), was our negative control. Therefore, Total RNA was extracted from spleen and GH3/B6 CELLS in the presence of b-glucan (250mgr/ml). Then, RT-PCR and PCR methods were done for verifying the expression of DECTIN-1. Our result showed that 150 and 250 μgr/ml concentrations of b-glucan are capable to stimulation PROLACTIN synthesis significantly from GH3/B6 CELLS in comparison with control(P<0.05). The results showed that spleen, the positive control, expressed DECTIN-1 as expected. Our results also showed that DECTIN-1 was expressed in GH3/B6 CELLS at very low level, and this expression was increased under the effect of b-glucan. Therefore, b-glucan may induce GH3/B6 CELLS throughout DECTIN-1 which lead increasing PROLACTIN secretion from these cells.

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APA: Copy

SHAERZADEH, F., SEPEHRI, H., HOSSEIN, GH., GOLIAEI, B., DELPHI, L., & RASSOULI, Y.. (2011). THE EFFECT OF DIFFERENT CONCENTRATIONS OF β- GLUCAN ON INTRACELLULAR PROLACTIN SYNTHESIS IN GH3/B6 CELLS AND ITS RECEPTOR, DECTIN-1. IRANIAN JOURNAL OF BIOLOGY, 23(5), 663-671. SID. https://sid.ir/paper/21312/en

Vancouver: Copy

SHAERZADEH F., SEPEHRI H., HOSSEIN GH., GOLIAEI B., DELPHI L., RASSOULI Y.. THE EFFECT OF DIFFERENT CONCENTRATIONS OF β- GLUCAN ON INTRACELLULAR PROLACTIN SYNTHESIS IN GH3/B6 CELLS AND ITS RECEPTOR, DECTIN-1. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;23(5):663-671. Available from: https://sid.ir/paper/21312/en

IEEE: Copy

F. SHAERZADEH, H. SEPEHRI, GH. HOSSEIN, B. GOLIAEI, L. DELPHI, and Y. RASSOULI, “THE EFFECT OF DIFFERENT CONCENTRATIONS OF β- GLUCAN ON INTRACELLULAR PROLACTIN SYNTHESIS IN GH3/B6 CELLS AND ITS RECEPTOR, DECTIN-1,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 5, pp. 663–671, 2011, [Online]. Available: https://sid.ir/paper/21312/en

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