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Information Journal Paper

Title

CONSTRUCTION OF PLANT EXPRESSION VECTORS HARBORING CHITINASE (CHIT42) AND GLUCANASE (BGN13.1) GENES FROM TRICHODERMA SPECIES

Pages

  880-894

Abstract

 Pathogenic fungi are including the most important causes of damage in agricultural products. One way to combat FUNGAL DISEASES is using genetic engineering to transfer genes encode hydrolase enzymes, especially CHITINASE and GLUCANASE to crops.Antagonistic fungi such as TRICHODERMA, producing hydrolase enzymes CHITINASE and GLUCANASE, have a significant inhibitory effect on the growth of pathogenic fungi.Enzymes isolated from different species of these fungi are more effective than those isolated from other sources. In the present study, chit 42 and bgn 13.1 genes isolated from T. atroviride and T. virens, were used to make three PLANT EXPRESSION CONSTRUCTS consists of two constructs harboring one CHITINASE gene (pBIKE1) or GLUCANASE gene (pBIKE2) and a construct harboring both of these genes (pBIKE3) under the control of CaMV35S promoter and NOS terminator. Also during the construction of these vectors, new vector which was named pBI121GUS- was developed based on pBI121, without the gusgene. Constructs in this research project can be transferred to plants to produce GM plants resistant against fungi or to analyze the effects of these genes on pathogens.

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    APA: Copy

    ESFAHANI, K., MOTALLEBI, M., & ZAMANI, M.R.. (2011). CONSTRUCTION OF PLANT EXPRESSION VECTORS HARBORING CHITINASE (CHIT42) AND GLUCANASE (BGN13.1) GENES FROM TRICHODERMA SPECIES. IRANIAN JOURNAL OF BIOLOGY, 24(6), 880-894. SID. https://sid.ir/paper/21332/en

    Vancouver: Copy

    ESFAHANI K., MOTALLEBI M., ZAMANI M.R.. CONSTRUCTION OF PLANT EXPRESSION VECTORS HARBORING CHITINASE (CHIT42) AND GLUCANASE (BGN13.1) GENES FROM TRICHODERMA SPECIES. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;24(6):880-894. Available from: https://sid.ir/paper/21332/en

    IEEE: Copy

    K. ESFAHANI, M. MOTALLEBI, and M.R. ZAMANI, “CONSTRUCTION OF PLANT EXPRESSION VECTORS HARBORING CHITINASE (CHIT42) AND GLUCANASE (BGN13.1) GENES FROM TRICHODERMA SPECIES,” IRANIAN JOURNAL OF BIOLOGY, vol. 24, no. 6, pp. 880–894, 2011, [Online]. Available: https://sid.ir/paper/21332/en

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