STUDY OF THE ROLE OF C-TERMINAL HDEL SIGNAL SEQUENCE OF PROTEINS USING IMMUNOGOLDLABELING OF TRANSGENIC AND WILD TOBACCO CELLS

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MOVAFEGHI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2006 | Volume:19 | Issue:3 Page(s): 282-289

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Title

STUDY OF THE ROLE OF C-TERMINAL HDEL SIGNAL SEQUENCE OF PROTEINS USING IMMUNOGOLDLABELING OF TRANSGENIC AND WILD TOBACCO CELLS

Author(s)

MOVAFEGHI A. | Issue Writer Certificate

Keywords

ERQ3

GOLGI COMPLEXQ3

HDEL SEQUENCEQ3

IMMUNOCYTOCHEMICAL LABELINGQ3

Abstract

Without any doubt, the issue of ER export is far from settled and discussions have been ongoing for almost two decades. Two models are currently competing for genERal acceptance. One model describes ER export as a non-selective diffusion into antERograde ER-dERived transport vesicles (bulk-flow). The second model postulates that proteins are actively selected and enriched during ER export (active transport). HowevER, the retrieval of HDEL-tagged proteins from the GOLGI COMPLEX is well established in diffERent cells. The secretion of ER residents is vERy low, and the true level of ER export is masked by degradation in a post-ER. To ovERcome this problem, we used two transformed tobacco plants, which produce barley alpha-amylase and alpha-amylase-HDEL as soluble cargo molecules. Labeling using cryo-sectioning and alpha-amylase antibodies revealed that in both transgenic plant cells the secreted molecule was distributed in the ER, cistERnae of the Golgi apparatus and cell wall. HowevER, tagging alpha-amylase with HDEL led to predominant labeling of the cis- and cis-most cistERnae. Occasional labeling of the compartments distal to the cis-Golgi in alpha-amylase-HDEL–producing plants indicated that saturation of ER retention may have occurred. In addition, both alpha-amylase and alpha-amylase-HDEL wERe detected in the cell wall, confirming that ER retention via HDEL tagging in the transformed cells was incomplete. Labeling of wild root cells with Calreticulin and Bip antibodies showed that this retrieval mechanism in plants has little impact on ER retention of soluble reticuloplasmins.

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APA: Copy

MOVAFEGHI, A.. (2006). STUDY OF THE ROLE OF C-TERMINAL HDEL SIGNAL SEQUENCE OF PROTEINS USING IMMUNOGOLDLABELING OF TRANSGENIC AND WILD TOBACCO CELLS . IRANIAN JOURNAL OF BIOLOGY, 19(3), 282-289. SID. https://sid.ir/paper/21386/en

Vancouver: Copy

MOVAFEGHI A.. STUDY OF THE ROLE OF C-TERMINAL HDEL SIGNAL SEQUENCE OF PROTEINS USING IMMUNOGOLDLABELING OF TRANSGENIC AND WILD TOBACCO CELLS . IRANIAN JOURNAL OF BIOLOGY[Internet]. 2006;19(3):282-289. Available from: https://sid.ir/paper/21386/en

IEEE: Copy

A. MOVAFEGHI, “STUDY OF THE ROLE OF C-TERMINAL HDEL SIGNAL SEQUENCE OF PROTEINS USING IMMUNOGOLDLABELING OF TRANSGENIC AND WILD TOBACCO CELLS ,” IRANIAN JOURNAL OF BIOLOGY, vol. 19, no. 3, pp. 282–289, 2006, [Online]. Available: https://sid.ir/paper/21386/en

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