STUDY OF APOPTOTIC ACTIVITY OF HYBRID PROTEIN (AI-GM-CSF) ON THE DIFFERENT CELL LINES BEARING GM-CSF RECEPTOR

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OLOUMI MANA; SHARIATI E.S.; BOUZARI S.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2009 | Volume:22 | Issue:1 Page(s): 85-94

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Title

STUDY OF APOPTOTIC ACTIVITY OF HYBRID PROTEIN (AI-GM-CSF) ON THE DIFFERENT CELL LINES BEARING GM-CSF RECEPTOR

Author(s)

OLOUMI MANA | SHARIATI E.S. | BOUZARI S. | Issue Writer Certificate

Keywords

APOPTOSISQ3

CANCERQ2

IMMUNOTOXINQ1

GM-CSFQ2

Abstract

One of the new approaches for targeted therapy of human disease like CANCER is the use of molecules so called IMMUNOTOXINs. IMMUNOTOXINs, comprised of both the cell targeting and the cell killing moieties. In the last few years a new approach for targeted therapy of human disease has been developed using cytotoxic molecules that are produced by gene fusion techniques. This class of molecules termed chimeric proteins comprises both the cell targeting and the cell killing moieties. In this investigation, the already constructed chimeric protein containing catalytic domain of Shiga-like toxin (Al) fused to human granulocyte macrophage colony stimulating factor (hGM-CSF) gene and cloned in E. coli was expressed by arabinose induction. The expressed protein extracted and then was analyzed for its cytotoxic activity on the cells bearing GM-CSF receptor. To elucidate the apoptotic activity of hybrid protein, the expressed protein was added to the cells and the apoptotic activity was measured by cellular DNA fragmentation ELISA kit (Roche). The results indicated that the chimeric protein was cytotoxic for the cells bearing GM-CSF receptor and it was shown that different concentration of the chimeric protein induced APOPTOSIS on the cells measured by the kit. The overall results indicated that cytotoxic activity of the chimeric protein is specific on the cells and it can also induce APOPTOSIS.

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APA: Copy

OLOUMI, MANA, SHARIATI, E.S., & BOUZARI, S.. (2009). STUDY OF APOPTOTIC ACTIVITY OF HYBRID PROTEIN (AI-GM-CSF) ON THE DIFFERENT CELL LINES BEARING GM-CSF RECEPTOR. IRANIAN JOURNAL OF BIOLOGY, 22(1), 85-94. SID. https://sid.ir/paper/21429/en

Vancouver: Copy

OLOUMI MANA, SHARIATI E.S., BOUZARI S.. STUDY OF APOPTOTIC ACTIVITY OF HYBRID PROTEIN (AI-GM-CSF) ON THE DIFFERENT CELL LINES BEARING GM-CSF RECEPTOR. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2009;22(1):85-94. Available from: https://sid.ir/paper/21429/en

IEEE: Copy

MANA OLOUMI, E.S. SHARIATI, and S. BOUZARI, “STUDY OF APOPTOTIC ACTIVITY OF HYBRID PROTEIN (AI-GM-CSF) ON THE DIFFERENT CELL LINES BEARING GM-CSF RECEPTOR,” IRANIAN JOURNAL OF BIOLOGY, vol. 22, no. 1, pp. 85–94, 2009, [Online]. Available: https://sid.ir/paper/21429/en

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