GENETIC GROUPING OF CERTAIN INDIGENOUS RHIZOBIAL STRAINS BY 16S- 23S IGS PCR-RFLP AND PLASMID PROFILE

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ALI KHANI H.A.; YAKHCHALI B.; ANTOVAN H.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2007 | Volume:20 | Issue:2 Page(s): 233-246

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Title

GENETIC GROUPING OF CERTAIN INDIGENOUS RHIZOBIAL STRAINS BY 16S- 23S IGS PCR-RFLP AND PLASMID PROFILE

Author(s)

ALI KHANI H.A. | YAKHCHALI B. | ANTOVAN H. | Issue Writer Certificate

Keywords

RIHZOBIL STRAINS 16S-23S IGS PCR-RFLPQ3

PLASMID PROFILEQ3

GENETIC GROUPING PGPRQ3

Abstract

Among prokaryotes, there are certain soil bacteria known as rhizobacteria which are very significant owing to their N2 fixation ability in legumes. The nucleotide sequence of the intergenic spacer (IGS) fragments residing between the 16S and 23S rRNA genes (16S-23S IGS) are not very conserved and thus are very useful in the grouping of rhizobial strains. In this research, 52 superior plant growth promoting rhizobacteria (PGPR) were grouped based on 16S-23S IGS PCR-RFLP and PLASMID PROFILEs. The results showed that the 16S- 23S IGS fragments of different rhizobial strains vary in size (600-1486 bp) and the number of IGS copies (1-3 copies). Based on the 16S-23S IGS PCR-RFLP profiles all 52 strains were located in 48 groups with 11 groups showing 70% intraspecies similarity. By using the above method, 77% of the strains (except 12 strains Bj 53, Bj 54; Rlv 27, Rlv 28, Rlv 23, Rlv 24; Rlp 16, Rlp 17; Sm 10, Sm 11, Sm 12 and Sm13) were separated into different groups. Therefore, this simple method which possesses minimal lab requirements is highly reliable and applicable for the genetic grouping of rhizobacteria in comparison to other molecular methods. The grouping based on the PLASMID PROFILE of rhizobial strains was in agreement (to some extent) with the results of the 16S-23S IGS PCR-RFLP method. For example, grouping of the Bj 53, Bj 54; Rlv 23, Rlv 24; Rlp 16, Rlp 17 and Sm 11, Sm 12 and Sm13 strains in both methods were completely similar and located in separate cluster groups.

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APA: Copy

ALI KHANI, H.A., YAKHCHALI, B., & ANTOVAN, H.. (2007). GENETIC GROUPING OF CERTAIN INDIGENOUS RHIZOBIAL STRAINS BY 16S- 23S IGS PCR-RFLP AND PLASMID PROFILE. IRANIAN JOURNAL OF BIOLOGY, 20(2), 233-246. SID. https://sid.ir/paper/21522/en

Vancouver: Copy

ALI KHANI H.A., YAKHCHALI B., ANTOVAN H.. GENETIC GROUPING OF CERTAIN INDIGENOUS RHIZOBIAL STRAINS BY 16S- 23S IGS PCR-RFLP AND PLASMID PROFILE. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2007;20(2):233-246. Available from: https://sid.ir/paper/21522/en

IEEE: Copy

H.A. ALI KHANI, B. YAKHCHALI, and H. ANTOVAN, “GENETIC GROUPING OF CERTAIN INDIGENOUS RHIZOBIAL STRAINS BY 16S- 23S IGS PCR-RFLP AND PLASMID PROFILE,” IRANIAN JOURNAL OF BIOLOGY, vol. 20, no. 2, pp. 233–246, 2007, [Online]. Available: https://sid.ir/paper/21522/en

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