DETERMINATION OF OPTIMUM GROWTH CONDITIONS FOR HALOARCULA IRU1 ISOLATED FROM UROMIA LAKE BY TAGUCHI METHOD

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AMIRKHANI H.; ASGARANI E.; KHODABANDEH M.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2012 | Volume:25 | Issue:1 Page(s): 148-156

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Title

DETERMINATION OF OPTIMUM GROWTH CONDITIONS FOR HALOARCULA IRU1 ISOLATED FROM UROMIA LAKE BY TAGUCHI METHOD

Author(s)

AMIRKHANI H. | ASGARANI E. | KHODABANDEH M. | Issue Writer Certificate

Keywords

HALOARCULAQ2

EXTREME HALOPHILEQ2

UROMIA SALT LAKE- GROWTH ASSESSMENTQ2

TAGUCHI METHODQ2

Abstract

HALOARCULA sp. IRU1 is an extremely halophilic archaeon which isolated from Uromia salt lake water. It exhibits high resistance to ionizing and ultra violet radiation and hydrogen peroxide. In this study, optimization of media components, pH, temperature and sodium chloride concentration for the growth and biomass production of HALOARCULAsp. IRU1 was carried out using TAGUCHI METHOD. By considering the Taguchi orthogonal array tables at 5 factors and 4 levels, 16 trials were designed. For each trial, sampling was performed twice immediately after inoculation and optical density (OD) was measured by the spectrophotometer at 600 nm. In the way, bacterial growth curve for each repeat of every trial was designed. In all of the trials, the concentration of inoculum was 5% and aeration was carried out at 220 rpm. Cell dry weights (grams per litre) of the above mentioned conditions were compared together by the Qualitek-4 program. The results showed that temperature had the most effect on the growth among several factors and there was no direct relation between sodium chloride concentration and culture medium composition. The optimum conditions obtained were pH 7; temperature 47oC; culture medium B; NaCl 20% (w/v). In these optimal conditions, the obtained cell concentration of 0.023 g.L-1 dry weight.h-1 was in agreement with predicted cell concentration.

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APA: Copy

AMIRKHANI, H., ASGARANI, E., & KHODABANDEH, M.. (2012). DETERMINATION OF OPTIMUM GROWTH CONDITIONS FOR HALOARCULA IRU1 ISOLATED FROM UROMIA LAKE BY TAGUCHI METHOD. IRANIAN JOURNAL OF BIOLOGY, 25(1), 148-156. SID. https://sid.ir/paper/21546/en

Vancouver: Copy

AMIRKHANI H., ASGARANI E., KHODABANDEH M.. DETERMINATION OF OPTIMUM GROWTH CONDITIONS FOR HALOARCULA IRU1 ISOLATED FROM UROMIA LAKE BY TAGUCHI METHOD. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2012;25(1):148-156. Available from: https://sid.ir/paper/21546/en

IEEE: Copy

H. AMIRKHANI, E. ASGARANI, and M. KHODABANDEH, “DETERMINATION OF OPTIMUM GROWTH CONDITIONS FOR HALOARCULA IRU1 ISOLATED FROM UROMIA LAKE BY TAGUCHI METHOD,” IRANIAN JOURNAL OF BIOLOGY, vol. 25, no. 1, pp. 148–156, 2012, [Online]. Available: https://sid.ir/paper/21546/en

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