REVIEW THE ABILITY OF BIOSURFACTANT PRODUCTION BY BACILLUS LICHENIFORMIS BCRC AND BACILLUS SUBTILIS HAZ2

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AKHAVAN SEPAHI A.; HAGHIGHAT S.; PASDAR H.

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Paper Information

Journal: JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT) Year:2009 | Volume:35 | Issue:1 (SECTION: BIOLOGY) Page(s): 67-74

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Title

REVIEW THE ABILITY OF BIOSURFACTANT PRODUCTION BY BACILLUS LICHENIFORMIS BCRC AND BACILLUS SUBTILIS HAZ2

Author(s)

AKHAVAN SEPAHI A. | HAGHIGHAT S. | PASDAR H. | Issue Writer Certificate

Keywords

MICROBIAL ENHANCED OIL RECOVERYQ3

BIOSURFACTANTQ2

BACILLUSQ3

SURFACE TENSIONQ3

LIPOPEPTIDEQ3

Abstract

MICROBIAL ENHANCED OIL RECOVERY (MEOR) is use of microorganisms in petroleum industry. BACILLUS species have been widely used as model organisms during MEOR research. In this study, isolation and identification of BIOSURFACTANT producing bacteria were assessed.60 strains of BACILLUS isolated from contaminated area close to the storage Masjed Soleiman and distribution center of oil products In Tehran refinery. The confirm the ability of isolates in BIOSURFACTANT production, haemolysis test, emulsification test and measurement of SURFACE TENSION were conducted. We also evaluated the effect of different pH, salinity concentration, temperatures and variety of carbon and nitrogen sources on BIOSURFACTANT production. Among important features of the isolated strains, two of the strains (P1, L2 BACILLUS sp.) showed high salt tolerance (8%) and their successful production of BIOSURFACTANT and reduce SURFACE TENSION was lowered from 69 to 30 and 29mN/m. The product of L2 and P1 is mainly LIPOPEPTIDE. These emulsified crude oil and other hydrocarbon P1, L2 BACILLUS sp. (E 24 = 90% -91%) respectively. These strains identified by morphological and biochemical technique using the taxonomic scheme of Bergey's manual of determinative bacteriology and 16SrRNA gene. The strains with approximate 97% and 99% belonged to BACILLUS subtilis and BACILLUS licheniformis. The optimum pH for these bacteria was 6.8 and the optimum temperature that they produce surfactant was 37oC .The best source of carbon was crude oil and best source of nitrogen was sodium nitrate. These strains in suitable for Exsitu MEOR.

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References

امکان سنجی ازدیاد برداشت میکروبی نفت (MEOR) بر روی مخازن نفتی ایران (کنگره)

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APA: Copy

AKHAVAN SEPAHI, A., HAGHIGHAT, S., & PASDAR, H.. (2009). REVIEW THE ABILITY OF BIOSURFACTANT PRODUCTION BY BACILLUS LICHENIFORMIS BCRC AND BACILLUS SUBTILIS HAZ2. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), 35(1 (SECTION: BIOLOGY)), 67-74. SID. https://sid.ir/paper/2164/en

Vancouver: Copy

AKHAVAN SEPAHI A., HAGHIGHAT S., PASDAR H.. REVIEW THE ABILITY OF BIOSURFACTANT PRODUCTION BY BACILLUS LICHENIFORMIS BCRC AND BACILLUS SUBTILIS HAZ2. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT)[Internet]. 2009;35(1 (SECTION: BIOLOGY)):67-74. Available from: https://sid.ir/paper/2164/en

IEEE: Copy

A. AKHAVAN SEPAHI, S. HAGHIGHAT, and H. PASDAR, “REVIEW THE ABILITY OF BIOSURFACTANT PRODUCTION BY BACILLUS LICHENIFORMIS BCRC AND BACILLUS SUBTILIS HAZ2,” JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), vol. 35, no. 1 (SECTION: BIOLOGY), pp. 67–74, 2009, [Online]. Available: https://sid.ir/paper/2164/en

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