Evaluation of regeneration potential of different layers of Amaryllis (Hippeastrum × johnsonii) bulb under influence of growth regulators

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Kharrazi Mahdiyeh; TEHRANIFAR ALI; SHARIFI AHMAD; BAGHERI ABDOLREZA

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2020 | Volume:11 | Issue:4 Page(s): 1-18

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Information Journal Paper

Title

Evaluation of regeneration potential of different layers of Amaryllis (Hippeastrum × johnsonii) bulb under influence of growth regulators

Author(s)

Kharrazi Mahdiyeh | TEHRANIFAR ALI | SHARIFI AHMAD | BAGHERI ABDOLREZA | Issue Writer Certificate

Keywords

bulb diameterQ1

BA hormoneQ1

culture mediumQ2

explantsQ2

Abstract

Objective Amaryllis is one of the ornamental bulbous plants, whose reproduction occurs slowly in natural conditions. Therefore, using tissue culture technique can be a suitable way to increase the multiplication rate of this ornamental plant. Material and methods In this study, a factorial experiment was conducted in a completely randomized design with 5 replications. The effect of different concentrations of BA and Kin (0, 0. 5, 1 and 2 mg/L) in combination with 0. 1 mg/L NAA on regeneration of different twin scale type of amaryllis was evaluated. explants were divided as twin scales and classified in 4 groups, so that the outermost twin scales was grouped in class 1 and the innermost twin scales was grouped in class 4. Results The results showed that the diameter of produced bulblet was influenced by the position of the twin scales in the mother bulb. The twin scale group one, which was made from the outer layers of the bulb, produced thicker bulblets, while the twin scales group four showed the minimum average of this trait. However, the explants cultured in a culture medium containing 0. 5 mg/l BA in combination with 0. 1 mg/l NAA had the highest bulblet diameter. Conclusion Generally, the application of twin scales group 1 and MS medium containing 0. 5 mg/l BA in combination with 0. 1 mg/l NAA is recommended for regeneration and proliferation of Amaryllis twin scale explants.

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APA: Copy

Kharrazi, Mahdiyeh, TEHRANIFAR, ALI, SHARIFI, AHMAD, & BAGHERI, ABDOLREZA. (2020). Evaluation of regeneration potential of different layers of Amaryllis (Hippeastrum × johnsonii) bulb under influence of growth regulators. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 11(4 ), 1-18. SID. https://sid.ir/paper/224550/en

Vancouver: Copy

Kharrazi Mahdiyeh, TEHRANIFAR ALI, SHARIFI AHMAD, BAGHERI ABDOLREZA. Evaluation of regeneration potential of different layers of Amaryllis (Hippeastrum × johnsonii) bulb under influence of growth regulators. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2020;11(4 ):1-18. Available from: https://sid.ir/paper/224550/en

IEEE: Copy

Mahdiyeh Kharrazi, ALI TEHRANIFAR, AHMAD SHARIFI, and ABDOLREZA BAGHERI, “Evaluation of regeneration potential of different layers of Amaryllis (Hippeastrum × johnsonii) bulb under influence of growth regulators,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 11, no. 4 , pp. 1–18, 2020, [Online]. Available: https://sid.ir/paper/224550/en

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