The effect of growth regulators and light on the phenolic content and in vitro regeneration of Aloe vera

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Saedi Amjad; MORADI HOSSEIN; KARIMI MAHNAZ

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NOVA BIOLOGICA REPERTA Year:2020 | Volume:6 | Issue:4 Page(s): 487-494

Download Full-Text

Persian Verion

View:

447

Download:

Cites:

Information Journal Paper

Title

The effect of growth regulators and light on the phenolic content and in vitro regeneration of Aloe vera

Author(s)

Saedi Amjad | MORADI HOSSEIN | KARIMI MAHNAZ | Issue Writer Certificate

Keywords

explantQ2

medicineQ1

mercuric chlorideQ1

stressQ2

tissue cultureQ2

Abstract

Aloe vera L. is one of the most valuable plants in the pharmaceutical, cosmetic, sanitary and food industries. In vitro culture is used for commercial production and due to the abundant application of this plant, extensive research has been performed on the in vitro culture of Aloe vera. For this purpose, the present study was conducted at two stages. At the first stage, the best method of sterilization of explants derived from Aloe vera offshoots was investigated. At the second stage, the effect of the type of explants, the light condition (dark and bright) and the effect of BAP (Benzyl Amino Purine) and NAA (α-Naphthalene acetic acid)) growth regulators on regeneration and the amount of phenolic compounds were studied. A factorial experiment was executed on the basis of a completely randomized design with three replications. The best sterilization protocol was 0. 1% mercuric chloride (for 2 minutes), 70% ethanol (for 30 seconds) and 15% sodium hypochlorite (for 5 minutes). The little white explant derived from the base of leaves, with the lowest percentage of phenol and the highest survival rate (67. 5%) in darkness, was found to be the best candidate. MS medium supplemented with 0. 75 mg / L BAP and 0. 25 mg / L NAA resulted in the highest stem number (2. 5) and stem length (42. 107 mm), establishment percentage (73%), leaf number (6. 33), leaf diameter (4. 8 mm), chlorophyll b (9. 216 mg/g) and carotenoids (4. 81 mg/g). The highest content of chlorophyll a (56. 07 mg/g) and total chlorophyll (61. 35 mg/g) were found in samples treated with hormonal medium, supplemented with 1. 5 mg / L of BAP with 0. 5 mg / L of NAA. The maximum number (3) and average length (33. 3 mm) of roots were observed in samples treated with the hormone-free medium.

Cites

No record.

References

No record.

Cite

APA: Copy

Saedi, Amjad, MORADI, HOSSEIN, & KARIMI, MAHNAZ. (2020). The effect of growth regulators and light on the phenolic content and in vitro regeneration of Aloe vera. NOVA BIOLOGICA REPERTA, 6(4 ), 487-494. SID. https://sid.ir/paper/250592/en

Vancouver: Copy

Saedi Amjad, MORADI HOSSEIN, KARIMI MAHNAZ. The effect of growth regulators and light on the phenolic content and in vitro regeneration of Aloe vera. NOVA BIOLOGICA REPERTA[Internet]. 2020;6(4 ):487-494. Available from: https://sid.ir/paper/250592/en

IEEE: Copy

Amjad Saedi, HOSSEIN MORADI, and MAHNAZ KARIMI, “The effect of growth regulators and light on the phenolic content and in vitro regeneration of Aloe vera,” NOVA BIOLOGICA REPERTA, vol. 6, no. 4 , pp. 487–494, 2020, [Online]. Available: https://sid.ir/paper/250592/en

Related Journal Papers