Optimization of Callogenesis and Regeneration of Cucumber (Cucumis Sativus L. ) UtilizingCotyledon, Hypocotyl and Leaf Explants

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ABDOLINASAB M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2018 | Volume:10 | Issue:2 Page(s): 75-92

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

578

Download:

Cites:

Information Journal Paper

Title

Optimization of Callogenesis and Regeneration of Cucumber (Cucumis Sativus L. ) UtilizingCotyledon, Hypocotyl and Leaf Explants

Author(s)

ABDOLINASAB M. | Issue Writer Certificate

Keywords

Micro propagationQ2

In vitro cultureQ1

CucumberQ3

NAAQ2

BAPQ2

Abstract

Supernia is one of the most important high-yield cultivars of the imported Cucumber hybrids. In order to optimize asexual propagation of this variety through In vitro culture, cotyledon, hypocotyl and leaf explants were placed in the MS basal medium containing 0, 0. 1, 0. 2, 0. 3 and 0. 4 mg/l concentrations of 1-Naphthaleneacetic acid (NAA) in combination with 1, 2, 3 and 4 mg/l of 6-Benzylaminopurine (BAP) hormone. The experiment was conducted as a factorial arrangement in completely randomized design at three replications and five samples in each replicate. The percentage of callogenesis, embryogenic calli and shoot frequency of the cultured explants were evaluated. The highest percentage of callogenesis (86. 6%) was obtained from cotyledon explant on the MS medium supplemented with 0. 3 mg/l NAA + 3 mg/l BAP hormones. The highest embryogenic calli (61. 6%) were developed on the MS medium supplemented with 0. 2 mg/l NAA + 2 mg/l BAP hormones in hypocotyl culture. Also, the maximum number of shoot (20. 08) were obtained on MS medium supplemented with 0. 3 mg/l NAA + 3 mg/l BAP hormones in hypocotyl explant. The regenerated shoots were transferred to half-strength MS medium supplemented with 1 mg/l NAA hormone for root induction and formation. Then, the grown seedlings were adapted to the in vivo condition. Based on the results of this study, it is recommended to use the cotyledon explant for propagation and regeneration of this genotype, especially in the gene transferring studies.

Cites

No record.

References

No record.

Cite

APA: Copy

ABDOLINASAB, M.. (2018). Optimization of Callogenesis and Regeneration of Cucumber (Cucumis Sativus L. ) UtilizingCotyledon, Hypocotyl and Leaf Explants. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 10(2 ), 75-92. SID. https://sid.ir/paper/224613/en

Vancouver: Copy

ABDOLINASAB M.. Optimization of Callogenesis and Regeneration of Cucumber (Cucumis Sativus L. ) UtilizingCotyledon, Hypocotyl and Leaf Explants. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2018;10(2 ):75-92. Available from: https://sid.ir/paper/224613/en

IEEE: Copy

M. ABDOLINASAB, “Optimization of Callogenesis and Regeneration of Cucumber (Cucumis Sativus L. ) UtilizingCotyledon, Hypocotyl and Leaf Explants,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 10, no. 2 , pp. 75–92, 2018, [Online]. Available: https://sid.ir/paper/224613/en

Related Journal Papers