STUDY OF PLANT REGENERATION TRAITS IN OBTAINED EMBRYOS FROM VARIOUS CONTAINERS IN MICROSPORES CULTURE OF RAPESEED (BRASSICA NAPUS L. CV. PF7045/91

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HADADI P.; MOEINI AHMAD; KARIMZADEH GHASEM; ABD ELAHI M.R.

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Paper Information

Journal: JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT) Year:2006 | Volume:32 | Issue:1 (SECTION: BIOLOGY 1) Page(s): 49-56

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Title

STUDY OF PLANT REGENERATION TRAITS IN OBTAINED EMBRYOS FROM VARIOUS CONTAINERS IN MICROSPORES CULTURE OF RAPESEED (BRASSICA NAPUS L. CV. PF7045/91

Author(s)

HADADI P. | MOEINI AHMAD | KARIMZADEH GHASEM | ABD ELAHI M.R. | Issue Writer Certificate

Keywords

RAPESEEDQ3

BRASSICA NAPUSQ3

CALLOGENIC EMBRYOSQ3

SECONDARY EMBRYOSQ3

REGENERATIONQ3

Abstract

Donor plants were grown in a growth chamber at a day/night temperature of 15/10oC (16/8 hours). Microspores at late-uninucleate to early bi-nucleate were isolated from buds 2.5-3.5 mm in length and were cultured in NLN-13 medium containing 13% sucrose. 30 days after culture of isolated microspores, cotyledonary embryos with 4-5 mm diameter were transferred to Bs medium in order to REGENERATION. Cultured embryos were put at 4oC for ten days with the purpose of cold stress, and then were transferred to growth chamber at temperature of 25oC. After 30 days 3 traits, the percentage of normal plantlets REGENERATION, callogenesis and secondary embryogenesis were investigated. The method of embryogenesis with 3 levels was experimental treatment, embryo production in 1 L flasks containing 100 ml culture medium, in 0.5 L. flasks containing 25 ml culture medium and in glass Petri dishes 100x15 mm containing 12/5 ml culture medium. The results showed significant differences (a= 1%) between produced embryos in various containers forpercentage of normal plantlets REGENERATION, callogenic embryo production and secondary embryogenesis. The comparison of means showed that 1 and 0.5 L. flasks were the best effective containers for normal plantlets production (group a, 58% and 54%, respectively). Embryos in 1 L flasks showed the least abnormal REGENERATION with 21.41% CALLOGENIC EMBRYOS and 11.11% SECONDARY EMBRYOS.

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APA: Copy

HADADI, P., MOEINI, AHMAD, KARIMZADEH, GHASEM, & ABD ELAHI, M.R.. (2006). STUDY OF PLANT REGENERATION TRAITS IN OBTAINED EMBRYOS FROM VARIOUS CONTAINERS IN MICROSPORES CULTURE OF RAPESEED (BRASSICA NAPUS L. CV. PF_{7045/91. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), 32(1 (SECTION: BIOLOGY 1)), 49-56. SID. https://sid.ir/paper/2248/en}

Vancouver: Copy

HADADI P., MOEINI AHMAD, KARIMZADEH GHASEM, ABD ELAHI M.R.. STUDY OF PLANT REGENERATION TRAITS IN OBTAINED EMBRYOS FROM VARIOUS CONTAINERS IN MICROSPORES CULTURE OF RAPESEED (BRASSICA NAPUS L. CV. PF_{7045/91. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT)[Internet]. 2006;32(1 (SECTION: BIOLOGY 1)):49-56. Available from: https://sid.ir/paper/2248/en}

IEEE: Copy

P. HADADI, AHMAD MOEINI, GHASEM KARIMZADEH, and M.R. ABD ELAHI, “STUDY OF PLANT REGENERATION TRAITS IN OBTAINED EMBRYOS FROM VARIOUS CONTAINERS IN MICROSPORES CULTURE OF RAPESEED (BRASSICA NAPUS L. CV. PF_{7045/91,” JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), vol. 32, no. 1 (SECTION: BIOLOGY 1), pp. 49–56, 2006, [Online]. Available: https://sid.ir/paper/2248/en}

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