MAPPING QTLS ASSOCIATED WITH WHEAT SEED GERMINATION UNDER NORMAL AND DROUGHT STRESS CONDITIONS

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KHALILI MAROUF; MOHAMMADI SEYED ABOLGHASEM

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Journal: Crop Biotechnology Year:2015 | Volume:4 | Issue:9 Page(s): 1-14

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Information Journal Paper

Title

MAPPING QTLS ASSOCIATED WITH WHEAT SEED GERMINATION UNDER NORMAL AND DROUGHT STRESS CONDITIONS

Author(s)

KHALILI MAROUF | MOHAMMADI SEYED ABOLGHASEM | Issue Writer Certificate

Keywords

QTLQ2

GERMINATION INDICESQ2

WHEATQ3

Abstract

Seed germination (capacity, time, and synchronization) is a manifest characteristic of the standard germination test of a cultivated variety. In order to identify genomic regions associated with seed germination, 149 bread WHEAT recombinant inbred lines and their parents RojoYecora and No. 49 were conducted in an alpha lattice design with two replications at the University of Mahabad during 2013-14, under normal and moisture stress conditions. Different traits including index of germination speed, final germination percentage, germination speed, germination rate, and average daily germination were measured. In this study, 340 pairs of microsatellite primers were used to determine the parental polymorphism. Furthermore, 7 retro transposon-based markers on LTR regions of barely retro transposons, and their twenty-eight combinations at IRAP technique, as well as, 63 combination of this primer with anchored ISSR primers at REMAP technique were used for population screening. QTL analysis using composite interval mapping (CIM) for each trait in each environment and mean of two environments were accomplished. For all studied traits, transgressive segregation was observed. In general, eleven QTLs were detected for all traits. Total phenotypic variance explained by these QTLs varied from 11.82 to 21.42 percent. The highest LOD value for QTL controlling germination speed (LOD=6.65) was obtained on chromosome 4BQSg-Normal. The results revealed that QTLs of index of germination rate and germination speed related to the quantity and quality of seed germination had desirable performance in all three environments. However, detected stable and cluster QTLs may be used in marker assisted selection (MAS) programs.

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APA: Copy

KHALILI, MAROUF, & MOHAMMADI, SEYED ABOLGHASEM. (2015). MAPPING QTLS ASSOCIATED WITH WHEAT SEED GERMINATION UNDER NORMAL AND DROUGHT STRESS CONDITIONS. JOURNAL OF CROP BIOTECHNOLOGY, 4(9), 1-14. SID. https://sid.ir/paper/226818/en

Vancouver: Copy

KHALILI MAROUF, MOHAMMADI SEYED ABOLGHASEM. MAPPING QTLS ASSOCIATED WITH WHEAT SEED GERMINATION UNDER NORMAL AND DROUGHT STRESS CONDITIONS. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2015;4(9):1-14. Available from: https://sid.ir/paper/226818/en

IEEE: Copy

MAROUF KHALILI, and SEYED ABOLGHASEM MOHAMMADI, “MAPPING QTLS ASSOCIATED WITH WHEAT SEED GERMINATION UNDER NORMAL AND DROUGHT STRESS CONDITIONS,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 4, no. 9, pp. 1–14, 2015, [Online]. Available: https://sid.ir/paper/226818/en

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