Screening and Isolation of Extracellular Lipase Producing Halophilic Bacteria Marinobacter sp. S-14 Isolated from Badab-e Surt Hypersaline Spring

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Satari Faghihi L.; AHMADY ASBCHIN S.; SEYEDALIPOUR B.; RIAZI GH.H.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF BIOTECHNOLOGY Year:2018 | Volume:9 | Issue:3 Page(s): 441-449

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

754

Download:

Cites:

Information Journal Paper

Title

Screening and Isolation of Extracellular Lipase Producing Halophilic Bacteria Marinobacter sp. S-14 Isolated from Badab-e Surt Hypersaline Spring

Author(s)

Satari Faghihi L. | AHMADY ASBCHIN S. | SEYEDALIPOUR B. | RIAZI GH.H. | Issue Writer Certificate

Keywords

Halophilic BacteriaQ1

Marinobacter sp.Q1

LipaseQ2

Hydrolysis enzymesQ1

Badab-e SurtQ1

Abstract

Aims Today, the ability to produce hydrolases enzyme that are active in high salt concentrations is considered a new approach to the use of Halophilic Bacteria in biotechnology. The aim of this study was the screening and isolation of extracellular Lipase producing Halophilic Bacteria Marinobacter sp. S-14 isolated from Badab-e Surt Hypersaline spring. Materials & Methods In this experimental study, 42 pure bacterial colonies were isolated from different samples of water, soil, sediment, and sludge from a hypersaline spring with a screening technique on the specific culture medium of Halophilic Bacteria. The isolate S-14, which showed the highest Lipase activity, was selected for the identification by biochemical methods and 16S rRNA gene analysis. In order to optimize the growth conditions of the isolate, considering the maximum time of bacterial growth (72 hours), temperature, salt concentration, pH, carbohydrate, and amino acid intake were examined. The results were edited by Chromas pro 2. 1. 1 software, and compared with EzTaxon database. Strains that were more similar to the isolate were identified. Sequence analysis of 16S rRNA were performed by BioEdit 7. 1. 9, Clustal-2X 2. 1, and MEGA 6, and the phylogenetic tree was drawn by the neighbor joining algorithm. Findings The isolate S-14 had 99% similarity to Marinobacter flavimaris and Marinobacter adhaerens. The isolate had optimum growth in 5% NaCl concentration, 35° C, and 7. 0 acidity. Conclusion The isolate S-14 can be an appropriate candidate to produce extracellular Lipase enzyme and can utilize Fructose and Phenylalanine as a sole source of carbon and energy.

Cites

No record.

References

No record.

Cite

APA: Copy

Satari Faghihi, L., AHMADY ASBCHIN, S., SEYEDALIPOUR, B., & RIAZI, GH.H.. (2018). Screening and Isolation of Extracellular Lipase Producing Halophilic Bacteria Marinobacter sp. S-14 Isolated from Badab-e Surt Hypersaline Spring. JOURNAL OF BIOTECHNOLOGY, 9(3 ), 441-449. SID. https://sid.ir/paper/231369/en

Vancouver: Copy

Satari Faghihi L., AHMADY ASBCHIN S., SEYEDALIPOUR B., RIAZI GH.H.. Screening and Isolation of Extracellular Lipase Producing Halophilic Bacteria Marinobacter sp. S-14 Isolated from Badab-e Surt Hypersaline Spring. JOURNAL OF BIOTECHNOLOGY[Internet]. 2018;9(3 ):441-449. Available from: https://sid.ir/paper/231369/en

IEEE: Copy

L. Satari Faghihi, S. AHMADY ASBCHIN, B. SEYEDALIPOUR, and GH.H. RIAZI, “Screening and Isolation of Extracellular Lipase Producing Halophilic Bacteria Marinobacter sp. S-14 Isolated from Badab-e Surt Hypersaline Spring,” JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 3 , pp. 441–449, 2018, [Online]. Available: https://sid.ir/paper/231369/en

Related Journal Papers