Effect of Electromagnetic Field and Nitric Oxide on the Neural Differentiation Proteins Marker and Viability of the Rat Bone Marrow Mesenchymal Stem Cells

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HAGHIGHAT N.; ABDOLMALEKI P.; BEHMANESH M.; Parnian J.

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2018 | Volume:9 | Issue:3 Page(s): 459-464

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Title

Effect of Electromagnetic Field and Nitric Oxide on the Neural Differentiation Proteins Marker and Viability of the Rat Bone Marrow Mesenchymal Stem Cells

Author(s)

HAGHIGHAT N. | ABDOLMALEKI P. | BEHMANESH M. | Parnian J. | Issue Writer Certificate

Keywords

Nitric OxideQ1

Electromagnetic FieldQ2

Mesenchymal Stem CellsQ2

MTTQ1

ImmunocytochemistryQ1

Abstract

Aims Nitric Oxide (NO) plays an important role in maintaining cellular stem status, and the range of Electromagnetic Fields (EMF) is very deep in contrast to the electric field. The aim of this study was to investigate the effect of Electromagnetic Field and Nitric Oxide on the neural differentiation proteins marker and viability of the rat bone marrow Mesenchymal Stem Cells. Materials & Methods The present experimental research was conducted on bone marrow Mesenchymal Stem Cells of Vistar rats. For treatments of the cells, high (1mM) and low (10micromolar Deta-NO) concentrations were used as a Nitric Oxide donor molecule and 50Hz low-frequency Electromagnetic Field and they were compared with the control group. The cell viability was recorded by MTT assay test, the neural differentiation pathway gene expression was investigated by RT-PCR technique, and the neural differentiation marker protein expression was evaluated by Immunocytochemistry technique. The data were analyzed by one-way ANOVA, using SPSS 13 software. Findings After 24 hours of treatment with Nitric Oxide and EMF, the rate of viability in all groups was significantly decreased compared to the control group. After 48 hours, EMF alone, as well as with low concentration of Nitric Oxide did not decrease the rate of viability and cell growth increased compared to the control group. In the group treated with high Nitric Oxide concentration along with EMF, MAP2 protein was expressed in the number of cells more than the control group and the one treated with EMF. Conclusion The Electromagnetic Field, along with its high concentration of Nitric Oxide, decreases the number of rat bone marrow Mesenchymal Stem Cells and, by increasing cell size, gene expression and neural differentiation proteins marker facilitates their differentiation to nerve-like cells.

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APA: Copy

HAGHIGHAT, N., ABDOLMALEKI, P., BEHMANESH, M., & Parnian, J.. (2018). Effect of Electromagnetic Field and Nitric Oxide on the Neural Differentiation Proteins Marker and Viability of the Rat Bone Marrow Mesenchymal Stem Cells. JOURNAL OF BIOTECHNOLOGY, 9(3 ), 459-464. SID. https://sid.ir/paper/231370/en

Vancouver: Copy

HAGHIGHAT N., ABDOLMALEKI P., BEHMANESH M., Parnian J.. Effect of Electromagnetic Field and Nitric Oxide on the Neural Differentiation Proteins Marker and Viability of the Rat Bone Marrow Mesenchymal Stem Cells. JOURNAL OF BIOTECHNOLOGY[Internet]. 2018;9(3 ):459-464. Available from: https://sid.ir/paper/231370/en

IEEE: Copy

N. HAGHIGHAT, P. ABDOLMALEKI, M. BEHMANESH, and J. Parnian, “Effect of Electromagnetic Field and Nitric Oxide on the Neural Differentiation Proteins Marker and Viability of the Rat Bone Marrow Mesenchymal Stem Cells,” JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 3 , pp. 459–464, 2018, [Online]. Available: https://sid.ir/paper/231370/en

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