THE OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION IN MEDICINAL LEMON VERBENA (LIPPIA CITRIODORA) PLANT

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TAHERI AZIZ ABADI HOJAT; BAGHERI NADALI; BABAEIAN JELODAR NADALI

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Journal: JOURNAL OF PLANT PROCESS AND FUNCTION Year:2016 | Volume:5 | Issue:17 Page(s): 53-62

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Title

THE OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION IN MEDICINAL LEMON VERBENA (LIPPIA CITRIODORA) PLANT

Author(s)

TAHERI AZIZ ABADI HOJAT | BAGHERI NADALI | BABAEIAN JELODAR NADALI | Issue Writer Certificate

Keywords

REGENERATIONQ2

LEMON VERBENAQ1

CALLUS INDUCTIONQ2

EXPLANTQ1

PLANT GROWTH REGULATORQ1

Abstract

This study was conducted to evaluate the effects of different types of growth regulators and their interaction on CALLUS INDUCTION and lemon REGENERATION in Lippia citriodora, using Murashige and Skoog (MS) medium. The CALLUS INDUCTION experiment with leaf and stem EXPLANTs was arranged in a factorial layout based on a completely randomized design. The experiment of lemon REGENERATION was performed in a completely randomized design as well. To evaluate of CALLUS INDUCTION, combination of 2, 4-Dichlorophenoxyacetic acid (2, 4-D) with 6-Benzylaminopurin (BAP) and with Naphthalene Acetic Acid (NAA) were applied. In REGENERATION study, combination of BAP with Kinetin (Kin) and with NAA were used. The effects of these PLANT GROWTH REGULATORs on CALLUS INDUCTION of leaf EXPLANT showed that treatment of 0 mg L-1 NAA and 2.0 mg L-1 2, 4-D had the highest (97%) rate of CALLUS INDUCTION. The highest percentage of callus formation was observed in stem EXPLANT at 0 mg L-1 NAA and 2.5 mg L-1 2, 4-D (97%) treatment. CALLUS INDUCTION in MS medium without PLANT GROWTH REGULATOR was not observed for leaf and stem EXPLANTs. The highest percentage of shoot production (40%) occurred in 1.0 mg L-1 BAP+0.05 mg L-1 Kin and the highest percentage of root production (40%) occurred when combined PLANT GROWTH REGULATOR of 1.0 mg L-1 BAP+0.05 mg L-1 NAA was used. According to these results, by using the medium containing the hormones BAP, 2, 4-D, NAA and Kin, in vitro culture of the L. citriodora can be used to fast and easy propagation of lemon.

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APA: Copy

TAHERI AZIZ ABADI, HOJAT, BAGHERI, NADALI, & BABAEIAN JELODAR, NADALI. (2016). THE OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION IN MEDICINAL LEMON VERBENA (LIPPIA CITRIODORA) PLANT. JOURNAL OF PLANT PROCESS AND FUNCTION, 5(17), 53-62. SID. https://sid.ir/paper/234194/en

Vancouver: Copy

TAHERI AZIZ ABADI HOJAT, BAGHERI NADALI, BABAEIAN JELODAR NADALI. THE OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION IN MEDICINAL LEMON VERBENA (LIPPIA CITRIODORA) PLANT. JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2016;5(17):53-62. Available from: https://sid.ir/paper/234194/en

IEEE: Copy

HOJAT TAHERI AZIZ ABADI, NADALI BAGHERI, and NADALI BABAEIAN JELODAR, “THE OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION IN MEDICINAL LEMON VERBENA (LIPPIA CITRIODORA) PLANT,” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 5, no. 17, pp. 53–62, 2016, [Online]. Available: https://sid.ir/paper/234194/en

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