OPTIMIZING LEAF CALLUS CULTURE IN SALVIA LERIIFOLIA FOR PHENOLIC ACIDS PRODUCTION

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MODARRES MASSOMEH; LAHOOTI MEHRDAD; ASILI JAVAD; KAFI MOHAMMAD; RAMAZANI ALI

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Journal: JOURNAL OF PLANT PROCESS AND FUNCTION Year:2013 | Volume:2 | Issue:2 Page(s): 65-73

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Title

OPTIMIZING LEAF CALLUS CULTURE IN SALVIA LERIIFOLIA FOR PHENOLIC ACIDS PRODUCTION

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Keywords

CALLUSQ2

P HENOLIC ACIDSQ2

SALVIA LERIIFOLIAQ1

Abstract

Salvia leriifolia is a medicinal plant from Lamiaceae family native to Khorasan and Semnan Provinaces. This plant has medicinal properties such as antinociceptive, anti-inflammatory, antidiabetic, antimicrobial and Antioxidant which all have been confirmed by researchers. In this research, CALLUS induction was established for production of phenolic acids. For this purpose, the leaf explants were cultured in MS medium supplemented with 2, 4-D (0, 1, 2, 3, 4 mgL-1), KIN (0, 0.3, 1mgL-1), BAP (0, 1, 2, 3, 4, 5, 6 mgL-1) and NAA (0, 1, 2, 3 mgL-1). Accumulation of phenolic acids was measured after 4 weeks of culture by HPLC. Results showed that the best treatments for CALLUS induction and growth were at the BAP5 mgl-1+NAA5mgl-1 and 2, 4- D 3 mgl-1+KIN1mgl-1. Accumulation of caffeic acid and salvianolic acid B in calli was higher than leaves of SALVIA LERIIFOLIAs. The highest rosmarinic acid concentration was found in the treatment BAP 5mgl-1+NAA5mgl-1 that were about 3 times higher than in leaves.

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APA: Copy

MODARRES, MASSOMEH, LAHOOTI, MEHRDAD, ASILI, JAVAD, KAFI, MOHAMMAD, & RAMAZANI, ALI. (2013). OPTIMIZING LEAF CALLUS CULTURE IN SALVIA LERIIFOLIA FOR PHENOLIC ACIDS PRODUCTION. JOURNAL OF PLANT PROCESS AND FUNCTION, 2(2), 65-73. SID. https://sid.ir/paper/234298/en

Vancouver: Copy

MODARRES MASSOMEH, LAHOOTI MEHRDAD, ASILI JAVAD, KAFI MOHAMMAD, RAMAZANI ALI. OPTIMIZING LEAF CALLUS CULTURE IN SALVIA LERIIFOLIA FOR PHENOLIC ACIDS PRODUCTION. JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2013;2(2):65-73. Available from: https://sid.ir/paper/234298/en

IEEE: Copy

MASSOMEH MODARRES, MEHRDAD LAHOOTI, JAVAD ASILI, MOHAMMAD KAFI, and ALI RAMAZANI, “OPTIMIZING LEAF CALLUS CULTURE IN SALVIA LERIIFOLIA FOR PHENOLIC ACIDS PRODUCTION,” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 2, no. 2, pp. 65–73, 2013, [Online]. Available: https://sid.ir/paper/234298/en

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