DESIGNING AND COMPARISON STUDY OF RAPID DETECTION METHODS OF RESISTANCE TO INJECTABLE DRUGS IN CLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SALEHI FATEMEH; RAHIMIYAN ZARIF BAHAREH; AHMADI AZAM; SHOJAPOUR MANA; POLAD TOKTAM; ARJOMANDZADEGAN MOHAMMAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Microbial Biology Year:2012 | Volume:1 | Issue:3 Page(s): 13-28

Download Full-Text

Persian Verion

View:

1,075

Download:

Cites:

Information Journal Paper

Title

DESIGNING AND COMPARISON STUDY OF RAPID DETECTION METHODS OF RESISTANCE TO INJECTABLE DRUGS IN CLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSIS

Author(s)

Keywords

MYCOBACTERIUM TUBERCULOSISQ2

KANAMYCIN AND AMIKACINQ2

RESISTANCEQ2

PCR-RFLPQ2

Abstract

Introduction: In this study, some molecular methods were designed for rapid detection of RESISTANCE to KANAMYCIN AND AMIKACIN.Materials and methods: Among 120 clinical isolates of MYCOBACTERIUM TUBERCULOSIS, 70 strains were selected for evaluation of possible mutations. A PCR-RFLP method was designed for detection of wild type (using enzyme ajii) and mutant from (BstFNI enzyme) of the isolates. Furthermore, allele specific method (as PCR) was designed for detection mutations in codons 1401 and 1402 gene rrs. Some selected isolates were sequenced.Results: In PCR-RFLP method, among the 70 strains examined by BstFNI enzyme, could detect 17 mutant strains among 24 phenotypicaly resistant and 44 non-mutant isolates from 46 susceptible isolates. The sensitivity of this method was %70.83 and specificity was %95.65 on the other hand, 12 mutant from 20 resistant strains and 29 non-mutant strains from 32 susceptible strains were detected by AjiI enzyme. The sensitivity and specificity of this method was 60 and %90.62, respectively. In MAS PCR, 3 mutants from 6 resistant strains and 12 non-mutants from 17 resistant strains were detected. The sensitivity of this method was 50 and specificity was 70.58. Results of sequencing method confirmed the results of molecular methods.Discussion and conclusion: PCR-RFLP method by BstFNI enzyme was the best method for rapid detection of MYCOBACTERIUM TUBERCULOSIS resistant to second-line injectable drugs and was recommended for routine use.

Cites

No record.

References

No record.

Cite

APA: Copy

SALEHI, FATEMEH, RAHIMIYAN ZARIF, BAHAREH, AHMADI, AZAM, SHOJAPOUR, MANA, POLAD, TOKTAM, & ARJOMANDZADEGAN, MOHAMMAD. (2012). DESIGNING AND COMPARISON STUDY OF RAPID DETECTION METHODS OF RESISTANCE TO INJECTABLE DRUGS IN CLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSIS. BIOLOGICAL JOURNAL OF MICROORGANISM, 1(3), 13-28. SID. https://sid.ir/paper/237283/en

Vancouver: Copy

SALEHI FATEMEH, RAHIMIYAN ZARIF BAHAREH, AHMADI AZAM, SHOJAPOUR MANA, POLAD TOKTAM, ARJOMANDZADEGAN MOHAMMAD. DESIGNING AND COMPARISON STUDY OF RAPID DETECTION METHODS OF RESISTANCE TO INJECTABLE DRUGS IN CLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSIS. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2012;1(3):13-28. Available from: https://sid.ir/paper/237283/en

IEEE: Copy

FATEMEH SALEHI, BAHAREH RAHIMIYAN ZARIF, AZAM AHMADI, MANA SHOJAPOUR, TOKTAM POLAD, and MOHAMMAD ARJOMANDZADEGAN, “DESIGNING AND COMPARISON STUDY OF RAPID DETECTION METHODS OF RESISTANCE TO INJECTABLE DRUGS IN CLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSIS,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 1, no. 3, pp. 13–28, 2012, [Online]. Available: https://sid.ir/paper/237283/en

Related Journal Papers