Genotyping and Detection of Klebsiella Pneumoniae Carbapenemase (KPC) Enzyme among Carbapenem-resistant Enterobacteriaceae Family isolated from Isfahan Hospitals

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHOKRI DARIUSH; FATEMI SEYED MASIH; MOHAMMADI SAMIRA; Karimi Sediqeh

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: BIOLOGICAL JOURNAL OF MICROORGANISM Year:2020 | Volume:8 | Issue:32 Page(s): 109-116

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

752

Download:

175

Cites:

Information Journal Paper

Title

Genotyping and Detection of Klebsiella Pneumoniae Carbapenemase (KPC) Enzyme among Carbapenem-resistant Enterobacteriaceae Family isolated from Isfahan Hospitals

Author(s)

SHOKRI DARIUSH | FATEMI SEYED MASIH | MOHAMMADI SAMIRA | Karimi Sediqeh | Issue Writer Certificate

Keywords

Escherichia coliQ1

EnterobacterQ2

CitrobacterQ1

carbapenemQ2

Klebsiella Pneumoniae Carbapenemase (KPC)Q1

Abstract

Introduction: Carbapenem-resistant Enterobacteriaceae (CRE) bacteria are difficult to treat because of their high antibiotic resistance levels that can be mediated by carbapenemase enzymes such as Klebsiella Pneumoniae Carbapenemase (KPC). The purposes of this study were to determine the genetic and resistance patterns and to detect of KPC enzyme in carbapenem-resistant strains of Enterobacteriaceae isolates. Materials and methods: In this study, antibiotic resistant pattern and genotyping of carbapenem-resistant Enterobacteriaceae isolates and frequency of KPC enzyme were investigated. During 16 months of conducting the study (December 2016 until April 2018), strains of Escherichia coli, Enterobacter spp. and Citrobacter spp. were isolated and identified from different clinical specimens and antibiotic susceptibility test was determined. In addition, the prevalence of the KPC enzyme was determined by PCR and two phenotypic methods including Modified Hodge Test (MHT) and the combination test by boronic acid. Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) method was used for the determination of the clonal relationship of carbapenems resistant strains. Results: The results showed that among 520, 146 and 58 isolated of Escherichia coli, Enterobacter spp. and Citrobacter spp. effective antibiotics were carbapenems, piperacillin/tazobactam, amikacin, cefepime, fosfomycin, nitrofurantoin, and gentamicin, respectively. In addition, 12 strains (2. 3%) of E. coli, 4 strains (2. 7%) of Enterobacter and 4 strains (6. 9%) of Citrobacter were resistant to carbapenems. The KPC investigation results showed the detection of this enzyme in 18 and 6 isolates using MHT and boronic acid methods respectively, but no producer strain was observed using PCR. The result of ERICPCR showed in carbapenem-resistant strains of Citrobacter, Enterobacter and Escherichia coli, 3, 4 and 9 distinct main clusters (patterns) were observed respectively. Discussion and conclusion: This study demonstrates the high antibiotic resistant prevalence and low specificity of standard phenotypic methods that were used for KPC detection in Isfahan City.

Cites

No record.

References

No record.

Cite

APA: Copy

SHOKRI, DARIUSH, FATEMI, SEYED MASIH, MOHAMMADI, SAMIRA, & Karimi, Sediqeh. (2020). Genotyping and Detection of Klebsiella Pneumoniae Carbapenemase (KPC) Enzyme among Carbapenem-resistant Enterobacteriaceae Family isolated from Isfahan Hospitals. BIOLOGICAL JOURNAL OF MICROORGANISM, 8(32 ), 109-116. SID. https://sid.ir/paper/237323/en

Vancouver: Copy

SHOKRI DARIUSH, FATEMI SEYED MASIH, MOHAMMADI SAMIRA, Karimi Sediqeh. Genotyping and Detection of Klebsiella Pneumoniae Carbapenemase (KPC) Enzyme among Carbapenem-resistant Enterobacteriaceae Family isolated from Isfahan Hospitals. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2020;8(32 ):109-116. Available from: https://sid.ir/paper/237323/en

IEEE: Copy

DARIUSH SHOKRI, SEYED MASIH FATEMI, SAMIRA MOHAMMADI, and Sediqeh Karimi, “Genotyping and Detection of Klebsiella Pneumoniae Carbapenemase (KPC) Enzyme among Carbapenem-resistant Enterobacteriaceae Family isolated from Isfahan Hospitals,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 8, no. 32 , pp. 109–116, 2020, [Online]. Available: https://sid.ir/paper/237323/en

Related Journal Papers