Isolation and identification of vinblastine from the fungus of Chaetomium globosum Cr95 isolated from Catharanthus roseus plant

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ZAFARI DOUSTMORAD; Leylaiee Sahar; TAJICK MOHAMMAD ALI

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Journal: Journal of Microbial Biology Year:2020 | Volume:8 | Issue:32 Page(s): 1-14

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Information Journal Paper

Title

Isolation and identification of vinblastine from the fungus of Chaetomium globosum Cr95 isolated from Catharanthus roseus plant

Author(s)

ZAFARI DOUSTMORAD | Leylaiee Sahar | TAJICK MOHAMMAD ALI | Issue Writer Certificate

Keywords

Endophytic FungiQ1

Chaetomium globosumQ1

VinblastineQ1

Catharanthus roseusQ1

TIA PathwayQ1

Anti-proliferative ActivitiesQ1

Abstract

Introduction: Endophytic Fungi are capable of synthesizing many secondary plant metabolites and as such make available new compounds for pharmaceutical investigation. Thus, the endophytic fungus Cr95 isolate was separated from the stem of Catharanthus roseus plant found in Iran and screened for the production of Vinblastine. Materials and methods: By considering its morphologic and genotypic properties, it became possible to identify this isolate, followed by grouping in the Chaetomium globosum species. Furthermore, the Anti-proliferative Activities of C. globosum Cr95 isolate against P. oryzae as a model fungus, were assessed. This endophytic fungus was screened for the production of vinca alkaloids using specific biochemical assays, tryptophan decarboxylase (TDC) encoding gene, and TLC and HPLC analyses. Results: The endophytic isolate was found to have significant cytotoxic effects and in the biochemical assays, C. globosum Cr 95 isolate showed a positive production of vinca alkaloids. TDC gene could be amplified from this isolate. The presence of Vinblastine in fungal culture filtrate was confirmed through chromatographic and spectroscopic analyses, and the amount was estimated to be 78 μ g/l. The cytotoxic activity of the partially purified fungal Vinblastine against conidia of P. oryzae was evaluated using tetrazolium salt MTT assay, and maximum susceptibility was found to occur with the IC 50 value of 5 μ g/ml. Discussion and conclusion: Fungal VBL in ethyl acetate extracts was characterized by TLC and HPLC. To the best of the authors’ knowledge, this is the first report on Vinblastine production from the endophytic fungus, C. globosum Cr 95 isolated from the C. roseus plant.

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APA: Copy

ZAFARI, DOUSTMORAD, Leylaiee, Sahar, & TAJICK, MOHAMMAD ALI. (2020). Isolation and identification of vinblastine from the fungus of Chaetomium globosum Cr95 isolated from Catharanthus roseus plant. BIOLOGICAL JOURNAL OF MICROORGANISM, 8(32 ), 1-14. SID. https://sid.ir/paper/237335/en

Vancouver: Copy

ZAFARI DOUSTMORAD, Leylaiee Sahar, TAJICK MOHAMMAD ALI. Isolation and identification of vinblastine from the fungus of Chaetomium globosum Cr95 isolated from Catharanthus roseus plant. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2020;8(32 ):1-14. Available from: https://sid.ir/paper/237335/en

IEEE: Copy

DOUSTMORAD ZAFARI, Sahar Leylaiee, and MOHAMMAD ALI TAJICK, “Isolation and identification of vinblastine from the fungus of Chaetomium globosum Cr95 isolated from Catharanthus roseus plant,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 8, no. 32 , pp. 1–14, 2020, [Online]. Available: https://sid.ir/paper/237335/en

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