CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA

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TAVAKOLI AREZOO; HAMZAH AINON

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Journal: BIOLOGICAL JOURNAL OF MICROORGANISM Year:2017 | Volume:5 | Issue:20 Page(s): 71-80

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Title

CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA

Author(s)

TAVAKOLI AREZOO | HAMZAH AINON | Issue Writer Certificate

Keywords

CATECHOL OXYGENASEQ1

DEGRADATIONQ2

TOLUENEQ2

ENZYME ASSAYQ2

Abstract

Introduction: Catechol is a common intermediate compound in aromatic DEGRADATION process. Some microorganisms have this potentiality to degrade aromatic hydrocarbons by catechol dioxygenases to less toxic compounds with ability of entering the tricarboxylic acid cycle. In the present study, the CATECHOL OXYGENASE activity was measured for 12 crude oil degrader bacteria.Materials and methods: CATECHOL OXYGENASE activity of two enzymes includes catechol 1, 2 dioxygenase and catechol 2, 3 dioxygenase were determined using spectrophotometer at 260 nm and 375 nm, respectively.Results: The highest enzyme activity for catechol 1, 2 dioxygenase by Bacillus cereus UKMP-6G was (0.07 U/mL) and about catechol 2, 3 dioxygenase was 0.031 U/mL by Rhodococcus ruber UKMP-5M during the first minute of incubation. Catechol 1, 2 dioxygenase and catechol 2, 3 dioxygenase followed the ortho and meta pathway, respectively.Discussion and conclusion: The ENZYME ASSAY results showed that among 12 examined bacteria, only R. ruber UKMP-5M has the ability to use meta pathway for DEGRADATION and produce 2-hydroxymuconic acid. The other isolates use ortho pathway and create cis, cis -muconic acid.

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APA: Copy

TAVAKOLI, AREZOO, & HAMZAH, AINON. (2017). CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA. BIOLOGICAL JOURNAL OF MICROORGANISM, 5(20 ), 71-80. SID. https://sid.ir/paper/237350/en

Vancouver: Copy

TAVAKOLI AREZOO, HAMZAH AINON. CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2017;5(20 ):71-80. Available from: https://sid.ir/paper/237350/en

IEEE: Copy

AREZOO TAVAKOLI, and AINON HAMZAH, “CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 5, no. 20 , pp. 71–80, 2017, [Online]. Available: https://sid.ir/paper/237350/en

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