ISOLATION OF BACILLUS SP PRODUCING POLYHYDROXYALKANOATE (PHA) FROM ISFAHAN REFINERY WASTEWATER AND QUALIFICATION OF PRODUCTION IN SUBMERGED FERMENTATION

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KESHAVARZ AZAM MAHSA; NAGHAVI NAFISEH SADAT; ETEMADIFAR ZAHRA

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Journal: BIOLOGICAL JOURNAL OF MICROORGANISM Year:2015 | Volume:4 | Issue:15 Page(s): 83-98

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Title

ISOLATION OF BACILLUS SP PRODUCING POLYHYDROXYALKANOATE (PHA) FROM ISFAHAN REFINERY WASTEWATER AND QUALIFICATION OF PRODUCTION IN SUBMERGED FERMENTATION

Author(s)

KESHAVARZ AZAM MAHSA | NAGHAVI NAFISEH SADAT | ETEMADIFAR ZAHRA | Issue Writer Certificate

Keywords

POLYHYDROXYBUTYRATEQ2

BIODEGRADABLE PLASTICSQ2

BACILLUSQ2

OPTIMIZED PRODUCTION CONDITIONSQ2

Abstract

Introduction: The aim of present study was isolation of POLYHYDROXYBUTYRATE producing BACILLUS species from oil refinery waste water, Isfahan, Iran and primarily optimization of production condition. Petroleum wastes are rich of carbon sources and have low amounts of nitrogen and phosphorus sources. AS the most important factor in production of intracellular inclusions is increasing the C/N ratio, it seemed that POLYHYDROXYBUTYRATE producing microorganisms will be found in these wastes.Materials and methods: BACILLUS species were isolated and purified from oil refinery wastewater. The polymer was verified using different staining procedures. Polymer was extracted by digestion method and the optimum production conditions were investigated in minimal salt medium with the organic carbon source by submerged fermentation. Production of POLYHYDROXYBUTYRATE was studied using dry weight and optical density measurement.Results: Between various isolated BACILLUS strains, two of them (B1 and B2) were POLYHYDROXYBUTYRATE producers. Maximum PHA production based on dry weight and concentration were obtained for strain B1 after 72 hours incubation, at 31oC, in the presence of glucose as carbon source and yeast extract as nitrogen source, pH=7, and aeration in 120 rpm; and for strain B2 in the same condition, except optimal temperature which was 32oC. The most production amounts were 367 mg.ml-1 for B1 and 473 mg.ml-1 for B2 isolates. Also the most polymer percentage was 52.16 and 58.43 for B1 and B2 isolates respectively.Discussion and conclusion: The results showed that the production of POLYHYDROXYBUTYRATE was increased by optimization of the conditions in both isolates. Using petroleum wastes as well as production of BIODEGRADABLE PLASTICS, leads to decontamination of theses wastes.

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APA: Copy

KESHAVARZ AZAM, MAHSA, NAGHAVI, NAFISEH SADAT, & ETEMADIFAR, ZAHRA. (2015). ISOLATION OF BACILLUS SP PRODUCING POLYHYDROXYALKANOATE (PHA) FROM ISFAHAN REFINERY WASTEWATER AND QUALIFICATION OF PRODUCTION IN SUBMERGED FERMENTATION. BIOLOGICAL JOURNAL OF MICROORGANISM, 4(15), 83-98. SID. https://sid.ir/paper/237383/en

Vancouver: Copy

KESHAVARZ AZAM MAHSA, NAGHAVI NAFISEH SADAT, ETEMADIFAR ZAHRA. ISOLATION OF BACILLUS SP PRODUCING POLYHYDROXYALKANOATE (PHA) FROM ISFAHAN REFINERY WASTEWATER AND QUALIFICATION OF PRODUCTION IN SUBMERGED FERMENTATION. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2015;4(15):83-98. Available from: https://sid.ir/paper/237383/en

IEEE: Copy

MAHSA KESHAVARZ AZAM, NAFISEH SADAT NAGHAVI, and ZAHRA ETEMADIFAR, “ISOLATION OF BACILLUS SP PRODUCING POLYHYDROXYALKANOATE (PHA) FROM ISFAHAN REFINERY WASTEWATER AND QUALIFICATION OF PRODUCTION IN SUBMERGED FERMENTATION,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 4, no. 15, pp. 83–98, 2015, [Online]. Available: https://sid.ir/paper/237383/en

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