MEASUREMENT OF ANTIMICROBIAL ACTIVITY OF ISOLATED BACTERIA FROM THE CASPIAN SEA AND MOLECULAR IDENTIFICATION OF STRAINS WITH ANTIMICROBIAL EFFECT

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HAROUNABADI SAJAD; SHOKOUHI MOSTAFAVI SEYYED KHALIL; EGHBALI SHAMSABAD PARVANEH; MEYBODI SEYYED MANSOUR

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Journal: BIOLOGICAL JOURNAL OF MICROORGANISM Year:2015 | Volume:4 | Issue:15 Page(s): 167-178

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Title

MEASUREMENT OF ANTIMICROBIAL ACTIVITY OF ISOLATED BACTERIA FROM THE CASPIAN SEA AND MOLECULAR IDENTIFICATION OF STRAINS WITH ANTIMICROBIAL EFFECT

Author(s)

HAROUNABADI SAJAD | SHOKOUHI MOSTAFAVI SEYYED KHALIL | EGHBALI SHAMSABAD PARVANEH | MEYBODI SEYYED MANSOUR | Issue Writer Certificate

Keywords

STREPTOMYCES GRISEUSQ2

CASPIAN SEAQ2

ANTIMICROBIAL ACTIVITYQ2

Abstract

Introduction: Easy access and wide use of antimicrobial compounds led to the emergence of resistance among microorganisms. Therefore, screening and identifying antimicrobial compound with high effect of microorganisms in different environments is necessary and vital . Using microorganisms for biological aims change them to an important tool to control pathogens. STREPTOMYCES GRISEUS is one of them. The aim of this study is isolation of marine bacteria with antimicrobial effect against gram positive and negative bacteria. Finally, molecular identification of strains with ANTIMICROBIAL ACTIVITY.Materials and methods: In this study, 162 strains were isolated from the CASPIAN SEA .The strains were cultured on special medium and finally ANTIMICROBIAL ACTIVITY on references strains as measured. Among them four strains with remarkable ANTIMICROBIAL ACTIVITY were identified and selected. The strains were subjected to 16S rDNA PCR sequencing. The strains were submitted to NCBI as new STREPTOMYCES GRISEUS strains.Results: Among 162 strains, 4 strains had the most ANTIMICROBIAL ACTIVITY. The result showed, the strains were the most effective on Bacillus subtilis and Staphylococcus aureus (Gram positive bacteria) and the least effect were observed on Escherichia coli and Pseudomonas aeruginosa (Gram negative bacteria). After sequencing, the strains were classified to sterptomyces griseus genu.Discussion and conclusion: In this study, 4 strains with ANTIMICROBIAL ACTIVITY were identified. According to the strength of these bacteria for controlling pathogenic bacteria resistant to antibiotic, we can have more pure microorganisms in optimized and controlled conditions for using in pharmaceutical industries and also for the treatment of dangerous pathogenic bacteria.

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APA: Copy

HAROUNABADI, SAJAD, SHOKOUHI MOSTAFAVI, SEYYED KHALIL, EGHBALI SHAMSABAD, PARVANEH, & MEYBODI, SEYYED MANSOUR. (2015). MEASUREMENT OF ANTIMICROBIAL ACTIVITY OF ISOLATED BACTERIA FROM THE CASPIAN SEA AND MOLECULAR IDENTIFICATION OF STRAINS WITH ANTIMICROBIAL EFFECT. BIOLOGICAL JOURNAL OF MICROORGANISM, 4(15), 167-178. SID. https://sid.ir/paper/237384/en

Vancouver: Copy

HAROUNABADI SAJAD, SHOKOUHI MOSTAFAVI SEYYED KHALIL, EGHBALI SHAMSABAD PARVANEH, MEYBODI SEYYED MANSOUR. MEASUREMENT OF ANTIMICROBIAL ACTIVITY OF ISOLATED BACTERIA FROM THE CASPIAN SEA AND MOLECULAR IDENTIFICATION OF STRAINS WITH ANTIMICROBIAL EFFECT. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2015;4(15):167-178. Available from: https://sid.ir/paper/237384/en

IEEE: Copy

SAJAD HAROUNABADI, SEYYED KHALIL SHOKOUHI MOSTAFAVI, PARVANEH EGHBALI SHAMSABAD, and SEYYED MANSOUR MEYBODI, “MEASUREMENT OF ANTIMICROBIAL ACTIVITY OF ISOLATED BACTERIA FROM THE CASPIAN SEA AND MOLECULAR IDENTIFICATION OF STRAINS WITH ANTIMICROBIAL EFFECT,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 4, no. 15, pp. 167–178, 2015, [Online]. Available: https://sid.ir/paper/237384/en

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