DETECTION OF VIBRIO SPECIES ISOLATED FROM ORNAMENTAL GUPPY FISH IN KASHAN, ISFAHAN, IRAN FISH CULTURING POUNDS

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KIANI SAMIRA; NAGHAVI NAFISEH SADAT; NAZARI ALIREZA

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Journal: Journal of Microbial Biology Year:2016 | Volume:4 | Issue:16 Page(s): 43-48

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Title

DETECTION OF VIBRIO SPECIES ISOLATED FROM ORNAMENTAL GUPPY FISH IN KASHAN, ISFAHAN, IRAN FISH CULTURING POUNDS

Author(s)

KIANI SAMIRA | NAGHAVI NAFISEH SADAT | NAZARI ALIREZA | Issue Writer Certificate

Keywords

VIBRIO SPP.Q2

ORNAMENTAL GUPPY FISHQ2

GENETIC ALIGNMENTQ2

WATER QUALITYQ2

Abstract

Introduction: Gram-negative bacteria are the most pathogenic bacteria for marine organisms including ornamental fish.Materials and methods: In the present study Vibrio species isolated from ORNAMENTAL GUPPY FISH in Kashan, Isfahan, Iran fish ponds and were detected according to molecular detection and GENETIC ALIGNMENT. Liver, kidney, skin, brain and gill samples were taken from ORNAMENTAL GUPPY FISH in Kashan, Isfahan, Iran. Samples were cultured on enriched culture media and purification steps were performed based on microbiological methods. Primary identification was done using biochemical characterization of the isolated bacteria. Molecular detection was done based on amplification of 16SrDNA sequence of Vibrio cholera genome containing ITS (internal transcribed spacer); and sequence alignment of the amplified nucleotides.Results: The isolated bacteria detected as VIBRIO SPP., including Vibrio cholera (99% sequence similarity), Vibrio alginolyticus, Vibrio mimicus and Vibrio parahaemolyticus (up to 90% similarity in the genome sequence). The aquaculture ponds had alkaline water and the amount of five-day BOD was not in a safe range, which are favorable conditions for Vibrio species.Discussion and conclusion: Aquatic organisms in Iran can be carriers of human pathogens such as Vibrio species. The results obtained in the present study and similar investigations should be mentioned in aquaculture healthcare systems.

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APA: Copy

KIANI, SAMIRA, NAGHAVI, NAFISEH SADAT, & NAZARI, ALIREZA. (2016). DETECTION OF VIBRIO SPECIES ISOLATED FROM ORNAMENTAL GUPPY FISH IN KASHAN, ISFAHAN, IRAN FISH CULTURING POUNDS. BIOLOGICAL JOURNAL OF MICROORGANISM, 4(16), 43-48. SID. https://sid.ir/paper/237467/en

Vancouver: Copy

KIANI SAMIRA, NAGHAVI NAFISEH SADAT, NAZARI ALIREZA. DETECTION OF VIBRIO SPECIES ISOLATED FROM ORNAMENTAL GUPPY FISH IN KASHAN, ISFAHAN, IRAN FISH CULTURING POUNDS. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2016;4(16):43-48. Available from: https://sid.ir/paper/237467/en

IEEE: Copy

SAMIRA KIANI, NAFISEH SADAT NAGHAVI, and ALIREZA NAZARI, “DETECTION OF VIBRIO SPECIES ISOLATED FROM ORNAMENTAL GUPPY FISH IN KASHAN, ISFAHAN, IRAN FISH CULTURING POUNDS,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 4, no. 16, pp. 43–48, 2016, [Online]. Available: https://sid.ir/paper/237467/en

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