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Information Journal Paper

Title

Extraction and Isolation of Three Genes of Saffron (Crocus sativus L. ) such as AREB, DREB and MPK to Drought Resistance

Pages

  251-267

Abstract

 Among environmental stresses, drought stress is one of the main stresses around the world. To mitigate the effects of environmental stresses, it is important to find genotypes that have genes and desirable traits. To this porpose, one of the most important objectives was to understand the molecular mechanisms associated with drought resistance in plants. With recent advances in molecular genetics, hundreds of genes that were induced by stress had been identified and used as gene candidates for genetic engineering. Saffron is a plant that grows in arid and semi-arid regions, and unlike many plants, it is a heroine with a different heat regime. The presence of numerous genes to deal with drought stress limitations in this plant could be investigated. By recognizing the genes involved in creating drought resistance and selecting suitable native varieties, saffron can produce relatively similar crops in most areas with different climatic conditions. In this study, the presence of AREB, DREB and MPK genes in saffron was proved for the first time. The patterns of expression of these key genes were investigated using Real Time-PCR technique in different tissues of saffron plant. Drought stresses were increased the expression of these genes. This increase in expression in plant and reproductive tissues was different, which may increase the final product of these genes and its resistance to drought. Their expression also had a correlation and mutual influence over each other. The protein structure of these genes had 366, 166, and 315 amino acids, respectively, which by examining this structure, the presence of a region of common acid in them, showed the coexistence of these three genes.

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    APA: Copy

    Yousefi Javan, Iman, & Gharari, Faezeh. (2019). Extraction and Isolation of Three Genes of Saffron (Crocus sativus L. ) such as AREB, DREB and MPK to Drought Resistance. JOURNAL OF SAFFRON RESEARCH, 6(2 ), 251-267. SID. https://sid.ir/paper/240416/en

    Vancouver: Copy

    Yousefi Javan Iman, Gharari Faezeh. Extraction and Isolation of Three Genes of Saffron (Crocus sativus L. ) such as AREB, DREB and MPK to Drought Resistance. JOURNAL OF SAFFRON RESEARCH[Internet]. 2019;6(2 ):251-267. Available from: https://sid.ir/paper/240416/en

    IEEE: Copy

    Iman Yousefi Javan, and Faezeh Gharari, “Extraction and Isolation of Three Genes of Saffron (Crocus sativus L. ) such as AREB, DREB and MPK to Drought Resistance,” JOURNAL OF SAFFRON RESEARCH, vol. 6, no. 2 , pp. 251–267, 2019, [Online]. Available: https://sid.ir/paper/240416/en

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