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Information Journal Paper

Title

Cormlet Production of Saffron (Crocus sativus L. ) using in vitro Culture Techniques

Pages

  179-189

Abstract

 Saffron is a triploid plant from Iridaceae family, which is propagated only via mother corms. In vitro culture techniques can accelerate the propagation of this plant in order to comply with the increasing demands of farmers to this important medicinal plant. The purpose of this research was to find out a proper protocol to cormlet production on the mother corms were collected in August and early September months which cold pretreated. To this end, two saffron ecotypes of corms were collected from Qaen and Souht Khorassan regions. Samples were kept at 3 1° C for 13 to 15 weeks. After sterilization, samples were transferred into half strength MS culture medium with 6% sucrose, 2 mg. l-1 IBA and 2, 4 and 6 mg. l-1 BAP based on a CRD with five replications. Control was without Growth regulators. The results showed that there were no significant differences between growth regulator concentrations in cormlet production. However, the August samples were significantly different in the number of cormlets per mother corm and cormlet diameter. According to the results, sufficient cold pre-treatment on corms harvested in August and culture in 1/2 MS medium without any Growth regulators are recommended for the production of micro-corms of saffron through in vitro culture, which is also economically important.

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  • Cite

    APA: Copy

    Izanloo, Ali, Derakhshan, Atefe, ALIZADEH, ZOHREH, & BEHDANI, MOHAMMAD ALI. (2019). Cormlet Production of Saffron (Crocus sativus L. ) using in vitro Culture Techniques. JOURNAL OF SAFFRON RESEARCH, 6(2 ), 179-189. SID. https://sid.ir/paper/240419/en

    Vancouver: Copy

    Izanloo Ali, Derakhshan Atefe, ALIZADEH ZOHREH, BEHDANI MOHAMMAD ALI. Cormlet Production of Saffron (Crocus sativus L. ) using in vitro Culture Techniques. JOURNAL OF SAFFRON RESEARCH[Internet]. 2019;6(2 ):179-189. Available from: https://sid.ir/paper/240419/en

    IEEE: Copy

    Ali Izanloo, Atefe Derakhshan, ZOHREH ALIZADEH, and MOHAMMAD ALI BEHDANI, “Cormlet Production of Saffron (Crocus sativus L. ) using in vitro Culture Techniques,” JOURNAL OF SAFFRON RESEARCH, vol. 6, no. 2 , pp. 179–189, 2019, [Online]. Available: https://sid.ir/paper/240419/en

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