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Information Journal Paper

Title

STUDY ON EFFECT OF TYPE OF EXPLANT AND HORMONE ON CALLUS INDUCTION AND REGENERATION IN SAFFRON (CROCUS SATIVUS L.)

Pages

  195-202

Abstract

 Saffron (Crocus sativus L.) is one of the medicinal plants that contain active components and medicinal materials. Tissue culture of SAFFRON can improve the quality and quantity of the SAFFRON product, increase its export and the farmers’ income.In this study, 36 different types of hormone combinations in the dark and 9 different treatments of hormone combinations in cold (4oC), using different SAFFRON explants (bulb, leaf, scales around leaf and distal parts of the leaf) were studied in tissue culture. To investigate the growth of corms, the callus formation and the regeneration rate, three replications for each treatment were used and the length of shoot (cm), the callus formation percentage and the regeneration percentage were measured and statistical analysis was performed. Among the types of explants, only explants from bulbs produced the callus on MS medium containing 2 mg.l-1 BAP and 1 mg.l-1 IBA in both the dark and cold conditions. The highest percentage of regeneration was obtained in MS medium with hormonal composition of 0.3 mg.l-1 TDZ, 1 mg.l-1 BAP, 2 mg.l-1 IBA and 0.01 mg.l-1 GA3 in the cold conditions.

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    APA: Copy

    Sajadi Fard, Mohsen, & Pazhouhandeh, Maghsoud. (2015). STUDY ON EFFECT OF TYPE OF EXPLANT AND HORMONE ON CALLUS INDUCTION AND REGENERATION IN SAFFRON (CROCUS SATIVUS L.). SAFFRON AGRONOMY AND TECHNOLOGY, 3(3), 195-202. SID. https://sid.ir/paper/247892/en

    Vancouver: Copy

    Sajadi Fard Mohsen, Pazhouhandeh Maghsoud. STUDY ON EFFECT OF TYPE OF EXPLANT AND HORMONE ON CALLUS INDUCTION AND REGENERATION IN SAFFRON (CROCUS SATIVUS L.). SAFFRON AGRONOMY AND TECHNOLOGY[Internet]. 2015;3(3):195-202. Available from: https://sid.ir/paper/247892/en

    IEEE: Copy

    Mohsen Sajadi Fard, and Maghsoud Pazhouhandeh, “STUDY ON EFFECT OF TYPE OF EXPLANT AND HORMONE ON CALLUS INDUCTION AND REGENERATION IN SAFFRON (CROCUS SATIVUS L.),” SAFFRON AGRONOMY AND TECHNOLOGY, vol. 3, no. 3, pp. 195–202, 2015, [Online]. Available: https://sid.ir/paper/247892/en

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