STUDY ON IRIDOVIRUS IN PERSIAN STURGEON (ACIPENSER PERSICUS) SPAWNERS USING A POLYMERASE CHAIN REACTION (PCR) ASSAY

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BAZARI MOGHADDAM S.; POURKAZEMI M.; SHENAVAR MASOULEH A.; HAGHIGHI KARSIDANI S.; CHAKMEHDOUZ GHASEMI F.

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Journal: Journal of Aquaculture Development Year:2013 | Volume:7 | Issue:2 Page(s): 1-7

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Title

STUDY ON IRIDOVIRUS IN PERSIAN STURGEON (ACIPENSER PERSICUS) SPAWNERS USING A POLYMERASE CHAIN REACTION (PCR) ASSAY

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Keywords

IRIDOVIRUSQ2

PERSIAN STURGEONQ2

CASPIAN SEAQ2

DNAQ2

PCRQ2

Abstract

IRIDOVIRUSes having five genera of IRIDOVIRUS, ChlorIRIDOVIRUS, Ranavirus, Lymphocystivirus and Megalocytivirus cause disease in many marine and freshwater fishes. This virus has been identified in different sturgeon species such as White sturgeon (Acipenser transmontanus), Paddlefish (Polyodon spathaula), Russian sturgeon (Acipenser guldenstaedtii) and Pallid sturgeon (Scaphirhynchus albus).Considering the importance of sturgeon species in CASPIAN SEA, this study carried out for the first time on PERSIAN STURGEON spawners caught in southern coasts of CASPIAN SEA. Since IRIDOVIRUS usually stay in epithelial cells of skin, especially in fins and reproduced quickly. We preserved 150 samples of pectoral fins in -20°c freezer for DNA extraction using IQ2000 identification kit. DNA extracted using produce as well as solutions provided by the sets of kit. The DNA quality was assessed using 1% agaroze gel electrophoresis PCR performed using suitable DNA and specific primers. In order to check the quality of PCR product 5ml of PCR product, electrophoresed by 2% agaroze gel and stained by Ethidium Bromide.Based on the sample presented on the kit, all the samples which have not carrying out IRIDOVIRUS, should produce 655 base pair PCR band and this band created in all samples. Therefore, according to results obtained in this investigation no IRIDOVIRUS has been detected in PERSIAN STURGEON brood stocks.

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APA: Copy

BAZARI MOGHADDAM, S., POURKAZEMI, M., SHENAVAR MASOULEH, A., HAGHIGHI KARSIDANI, S., & CHAKMEHDOUZ GHASEMI, F.. (2013). STUDY ON IRIDOVIRUS IN PERSIAN STURGEON (ACIPENSER PERSICUS) SPAWNERS USING A POLYMERASE CHAIN REACTION (PCR) ASSAY. JOURNAL OF AQUACULTURE DEVELOPMENT, 7(2), 1-7. SID. https://sid.ir/paper/242047/en

Vancouver: Copy

BAZARI MOGHADDAM S., POURKAZEMI M., SHENAVAR MASOULEH A., HAGHIGHI KARSIDANI S., CHAKMEHDOUZ GHASEMI F.. STUDY ON IRIDOVIRUS IN PERSIAN STURGEON (ACIPENSER PERSICUS) SPAWNERS USING A POLYMERASE CHAIN REACTION (PCR) ASSAY. JOURNAL OF AQUACULTURE DEVELOPMENT[Internet]. 2013;7(2):1-7. Available from: https://sid.ir/paper/242047/en

IEEE: Copy

S. BAZARI MOGHADDAM, M. POURKAZEMI, A. SHENAVAR MASOULEH, S. HAGHIGHI KARSIDANI, and F. CHAKMEHDOUZ GHASEMI, “STUDY ON IRIDOVIRUS IN PERSIAN STURGEON (ACIPENSER PERSICUS) SPAWNERS USING A POLYMERASE CHAIN REACTION (PCR) ASSAY,” JOURNAL OF AQUACULTURE DEVELOPMENT, vol. 7, no. 2, pp. 1–7, 2013, [Online]. Available: https://sid.ir/paper/242047/en

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