ISOLATION AND IDENTIFICATION OF GALACTOMANNOPROTEIN ASPERGILLUS FUMIGATUS AF-293

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SHAH HOSSEINI N.; KHABIRI A.; BAGHERI F.; ASSMAR M.; GHAEMI N.

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Journal: Biotechnological Journal of Environmental Microorganisms Year:2012 | Volume: | Issue: Page(s): 51-58

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Title

ISOLATION AND IDENTIFICATION OF GALACTOMANNOPROTEIN ASPERGILLUS FUMIGATUS AF-293

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Keywords

ASPERGILLUS SSP.Q2

GALACTOMANNOPROTEINQ2

ISOLATIONQ2

Abstract

Aspergillus spp are ubiquitous opportunistic moulds that cause both allergic and invasive syndromes. The genus comprises approximately 200 species, of which 18 have been associated with human disease. Glactomannan (GM) is an essential molecule in the life of the human opportunistic fungal pathogen Aspergillus fumigatus. GM is also produced in the host and is a well-known A. fumigatus antigen. The main aim of this study was identification of GALACTOMANNOPROTEIN, therefore A. fumigatus strain AF-293 was cultivated in Sabouraud’s liquid medium in three different conditions. After identification of GALACTOMANNOPROTEIN, samples were analyzed by sodium dodecyl sulfate polyacrylamide gel electrorhoresis (SDS-PAGE). GMPs stained with silver staining technique or electrotransferred to nitrocellulose. Serum samples from 15 patients were used through this study. Sera from patients were analyzed for the presence of antibody to A. fumigatus GMP by an indirect enzyme-linked immunosorbent assay (ELISA) method. Results illustrated that GMP had a molecular weight 60 kDa. In addition, ELISA results showed that GMP can interact with patients’ sera as an antigen.

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APA: Copy

SHAH HOSSEINI, N., KHABIRI, A., BAGHERI, F., ASSMAR, M., & GHAEMI, N.. (2012). ISOLATION AND IDENTIFICATION OF GALACTOMANNOPROTEIN ASPERGILLUS FUMIGATUS AF-293. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, 6(2 (2)), 51-58. SID. https://sid.ir/paper/242218/en

Vancouver: Copy

SHAH HOSSEINI N., KHABIRI A., BAGHERI F., ASSMAR M., GHAEMI N.. ISOLATION AND IDENTIFICATION OF GALACTOMANNOPROTEIN ASPERGILLUS FUMIGATUS AF-293. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS[Internet]. 2012;6(2 (2)):51-58. Available from: https://sid.ir/paper/242218/en

IEEE: Copy

N. SHAH HOSSEINI, A. KHABIRI, F. BAGHERI, M. ASSMAR, and N. GHAEMI, “ISOLATION AND IDENTIFICATION OF GALACTOMANNOPROTEIN ASPERGILLUS FUMIGATUS AF-293,” BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, vol. 6, no. 2 (2), pp. 51–58, 2012, [Online]. Available: https://sid.ir/paper/242218/en

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